Document Detail

Evidence for high affinity nickel transporter genes in heavy metal resistant Streptomyces spec.
MedLine Citation:
PMID:  11199488     Owner:  NLM     Status:  MEDLINE    
We have isolated 25 new strains of streptomycetes from soil samples of a polluted site at the former uranium mine, Wismut, in eastern Thuringia, Germany. The strains grew on medium containing 1 mM NiCl2 and thus were resistant to the heavy metal ion. Seven of the strains were further characterized. All of these strains were resistant to heavy metals in various degrees with up to 10 mM resistance against NiCl2 supplied with the liquid minimal growth medium. The high level of resistance prompted us to look for high affinity nickel transporter genes thought to provide a means to eliminate the excess nickel ions form the cells. Degenerate oligonucleotide primers derived from sequences of P-type ATPase transporter genes of Gram negative bacteria identified a fragment which shows deduced amino acid sequence similarities to known high affinity nickel transporters. Investigation of two genes obtained from the isolates Streptomyces spec. E8 and F4 showed high sequence divergence. This was unexpected since a transmissible plasmid had been thought to convey heavy metal resistance.
M J Amoroso; D Schubert; P Mitscherlich; P Schumann; E Kothe
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of basic microbiology     Volume:  40     ISSN:  0233-111X     ISO Abbreviation:  J. Basic Microbiol.     Publication Date:  2000  
Date Detail:
Created Date:  2001-01-29     Completed Date:  2001-09-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8503885     Medline TA:  J Basic Microbiol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  295-301     Citation Subset:  IM    
Friedrich-Schiller-Universität, Biologisch-Pharmazeutische Fakultät, Institut für Mikrobiologie, Mikrobielle Phytopathologie, Winzerlaer Str. 10, D-07745 Jena, Germany.
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MeSH Terms
Amino Acid Sequence
Antiporters / genetics
Bacterial Proteins / genetics*,  metabolism*
Base Sequence
Carrier Proteins / chemistry,  genetics*,  metabolism
Cation Transport Proteins*
Drug Resistance, Microbial
Membrane Proteins / chemistry,  genetics*,  metabolism
Metals, Heavy / pharmacology*
Microbial Sensitivity Tests
Molecular Sequence Data
Nickel / metabolism*,  pharmacology
Sequence Analysis, DNA
Soil Microbiology
Soil Pollutants
Streptomyces / drug effects*,  genetics,  metabolism
Reg. No./Substance:
0/Antiporters; 0/Bacterial Proteins; 0/Carrier Proteins; 0/Cation Transport Proteins; 0/HoxN protein, bacteria; 0/Membrane Proteins; 0/Metals, Heavy; 0/NixA protein, Helicobacter pylori; 0/Soil Pollutants; 7440-02-0/Nickel; 7440-61-1/Uranium

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