Document Detail

Evidence for a functional hepatocyte growth factor receptor in human mesangial cells.
MedLine Citation:
PMID:  9712174     Owner:  NLM     Status:  MEDLINE    
In this study we have investigated both the expression of c-met in cultured human mesangial cells and the proliferative effect of HGF on these cells. RNAse protection analysis using a c-met riboprobe showed c-met to be expressed and further that this expression was unaffected by the glucose concentration or osmolality of the media. Immunofluorescence studies performed using anti-HGF or anti-c-met antibodies clearly showed that both proteins are localised to human mesangial cells. Proliferation of human mesangial cells after 24-h treatment with HGF was also examined. HGF 10 ng/ml and 100 ng/ml stimulated 3-H-Thymidine incorporation 1.35-fold (P = 0.001) and 1.6-fold (P<0.00001) respectively in cells made quiescent for 24 h. A similar dose-dependent stimulation of proliferation was observed in cells made quiescent for 48 h. Finally, using RNAse protection analysis we have shown that HGF (10 ng/ml, 100 ng/ml) induces the expression of c-met in these cells in a dose-dependent manner. Together these results indicate for the first time a potential autocrine role for HGF in the human mesangium.
N C Kallincos; A N Pollard; J J Couper
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Regulatory peptides     Volume:  74     ISSN:  0167-0115     ISO Abbreviation:  Regul. Pept.     Publication Date:  1998 Jun 
Date Detail:
Created Date:  1998-11-06     Completed Date:  1998-11-06     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8100479     Medline TA:  Regul Pept     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  137-42     Citation Subset:  IM    
Department of Endocrinology, Women's and Children's Hospital, Adelaide, S.A. Australia.
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MeSH Terms
Cell Division
Cells, Cultured
Child, Preschool
Gene Expression
Glomerular Mesangium / cytology,  metabolism*
Hepatocyte Growth Factor / metabolism*,  pharmacology
Proto-Oncogene Proteins c-met / biosynthesis*,  genetics
Reg. No./Substance:
67256-21-7/Hepatocyte Growth Factor; EC Proteins c-met

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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