| Evidence from Serpula lacrymans that 2,5-dimethoxyhydroquinone is a lignocellulolytic agent of divergent brown-rot basidiomycetes. | |
| | |
MedLine Citation:
|
PMID: 23377930 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Basidiomycetes that cause brown rot of wood are essential biomass recyclers in coniferous forest ecosystems and a major cause of failure in wooden structures. Recent work indicates that distinct lineages of brown rot fungi have arisen independently from ligninolytic white rot ancestors via loss of lignocellulolytic enzymes. Brown rot thus proceeds without significant lignin removal, apparently beginning instead with oxidative attack on wood polymers by Fenton reagent produced when fungal hydroquinones or catechols reduce Fe(3+) in colonized wood. Since there is little evidence that white rot fungi produce these metabolites, one might infer that independent lineages of brown rot fungi would not employ the same Fe(3+) reductants. Recently, the catechol variegatic acid was proposed to drive Fenton chemistry in Serpula lacrymans, a brown rot member of the Boletales [Eastwood DC et al. (2011) Science 333:762-765]. We show that variegatic acid was undetectable in wood undergoing decay by S. lacrymans, that it was unable to reduce in vitro the Fe(3+) oxalate chelates that predominate in brown-rotting wood, and that it did not drive Fenton chemistry in vitro under physiological conditions. Instead, the decaying wood contained physiologically significant levels of 2,5-dimethoxyhydroquinone, a reductant with a demonstrated biodegradative role when wood is attacked by certain brown rot fungi in two other divergent lineages, the Gloeophyllales and the Polyporales. Our results suggest that the pathway for 2,5-dimethoxyhydroquinone biosynthesis may have been present in ancestral white rot basidiomycetes, but do not rule out the possibility that it appeared multiple times via convergent evolution. |
| | |
Authors:
|
Premsagar Korripally; Vitaliy I Timokhin; Carl J Houtman; Michael D Mozuch; Kenneth E Hammel |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2013-2-1 |
Journal Detail:
|
Title: Applied and environmental microbiology Volume: - ISSN: 1098-5336 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2013 Feb |
Date Detail:
|
Created Date: 2013-2-4 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Department of Biotechnology, Mahatma Gandhi University, Nalgonda 508254, India. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Genetic Determinants for Cadmium and Arsenic Resistance among Listeria monocytogenes Serotype 4b fro...
Next Document: Studies on a blasticidin S deaminase from Streptomyces lividans and its application in engineering a...