Document Detail

Evidence from Serpula lacrymans that 2,5-dimethoxyhydroquinone Is a lignocellulolytic agent of divergent brown rot basidiomycetes.
MedLine Citation:
PMID:  23377930     Owner:  NLM     Status:  MEDLINE    
Basidiomycetes that cause brown rot of wood are essential biomass recyclers in coniferous forest ecosystems and a major cause of failure in wooden structures. Recent work indicates that distinct lineages of brown rot fungi have arisen independently from ligninolytic white rot ancestors via loss of lignocellulolytic enzymes. Brown rot thus proceeds without significant lignin removal, apparently beginning instead with oxidative attack on wood polymers by Fenton reagent produced when fungal hydroquinones or catechols reduce Fe(3+) in colonized wood. Since there is little evidence that white rot fungi produce these metabolites, one question is the extent to which independent lineages of brown rot fungi may have evolved different Fe(3+) reductants. Recently, the catechol variegatic acid was proposed to drive Fenton chemistry in Serpula lacrymans, a brown rot member of the Boletales (D. C. Eastwood et al., Science 333:762-765, 2011). We found no variegatic acid in wood undergoing decay by S. lacrymans. We found also that variegatic acid failed to reduce in vitro the Fe(3+) oxalate chelates that predominate in brown-rotting wood and that it did not drive Fenton chemistry in vitro under physiological conditions. Instead, the decaying wood contained physiologically significant levels of 2,5-dimethoxyhydroquinone, a reductant with a demonstrated biodegradative role when wood is attacked by certain brown rot fungi in two other divergent lineages, the Gloeophyllales and Polyporales. Our results suggest that the pathway for 2,5-dimethoxyhydroquinone biosynthesis may have been present in ancestral white rot basidiomycetes but do not rule out the possibility that it appeared multiple times via convergent evolution.
Premsagar Korripally; Vitaliy I Timokhin; Carl J Houtman; Michael D Mozuch; Kenneth E Hammel
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2013-02-01
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  79     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-03-15     Completed Date:  2013-08-28     Revised Date:  2013-10-08    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2377-83     Citation Subset:  IM    
Department of Biotechnology, Mahatma Gandhi University, Nalgonda, India.
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MeSH Terms
Basidiomycota / metabolism*
Ferric Compounds / metabolism
Hydroquinones / metabolism*
Lignin / metabolism*
Metabolic Networks and Pathways
Wood / metabolism,  microbiology
Reg. No./Substance:
0/Ferric Compounds; 0/Hydroquinones; 11132-73-3/lignocellulose; 9005-53-2/Lignin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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