| Evidence for facilitated lactate uptake in lizard skeletal muscle. | |
| | |
MedLine Citation:
|
PMID: 11809784 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
To understand more fully lactate metabolism in reptilian muscle, lactate uptake in lizard skeletal muscle was measured and its similarities to the monocarboxylate transport system found in mammals were examined. At 2 min, uptake rates of 15 mmol l(-1) lactate into red iliofibularis (rIF) were 2.4- and 2.2-fold greater than white iliofibularis (wIF) and mouse soleus, respectively. alpha-Cyano-4-hydroxycinnamate (15 mmol l(-1)) caused little inhibition of uptake in wIF but caused a 42-54 % reduction in the uptake rate of lactate into rIF, suggesting that much of the lactate uptake by rIF is via protein-mediated transport. N-ethymaleimide (ETH) (10 mmol l(-1)) also caused a reduction in the rate of uptake, but measurements of adenylate and phosphocreatine concentrations show that ETH had serious effects on rIF and wIF and may not be appropriate for transport inhibition studies in reptiles. The higher net uptake rate by rIF than by wIF agrees with the fact that rIF shows much higher rates of lactate utilization and incorporation into glycogen than wIF. This study also suggests that lactate uptake by reptilian muscle is similar to that by mammalian muscle and that, evolutionarily, this transport system may be relatively conserved even in animals with very different patterns of lactate metabolism. |
| | |
Authors:
|
E R Donovan; T T Gleeson |
Related Documents
:
|
19762854 - Transport stress in broilers: i. blood metabolism, glycolytic potential, and meat quality. 23239154 - Critical illness myopathy and glut4 - significance of insulin and muscle contraction. 23371414 - Biological markers of exposure to organophosphorus nerve agents. 18566944 - Skeletal muscle changes after hemiparetic stroke and potential beneficial effects of ex... 15198864 - Muscle-nerve-muscle neurotization for the reinnervation of denervated somatic muscle. 8788164 - Dystrophin expression in muscles of mdx mice after adenovirus-mediated in vivo gene tra... |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
|
Title: The Journal of experimental biology Volume: 204 ISSN: 0022-0949 ISO Abbreviation: J. Exp. Biol. Publication Date: 2001 Dec |
Date Detail:
|
Created Date: 2002-01-25 Completed Date: 2002-02-28 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0243705 Medline TA: J Exp Biol Country: England |
Other Details:
|
Languages: eng Pagination: 4099-106 Citation Subset: IM |
Affiliation:
|
Section of Integrative Physiology and Neurobiology, E.P.O. Biology, University of Colorado-Boulder, Boulder, CO 80309-0334, USA. donovane@colorado.edu |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Ethylmaleimide / pharmacology Iguanas / metabolism* Kinetics Lactic Acid / metabolism* Mice Muscle Fibers, Fast-Twitch / drug effects, metabolism Muscle, Skeletal / drug effects, metabolism* |
| Chemical | |
Reg. No./Substance:
|
128-53-0/Ethylmaleimide; 50-21-5/Lactic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The diving behaviour of green turtles undertaking oceanic migration to and from Ascension Island: di...
Next Document: Simulated weightlessness alters the nycthemeral distribution of energy expenditure in rats.