Document Detail


Evidence for an expression of blood group A antigen on platelet glycoproteins IV and V.
MedLine Citation:
PMID:  8885154     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Blood group ABO antigens are known to be carried by several platelet glycoproteins (GP), e.g. GPIb, GPIIa, GPIIb, GPIIa and PECAM. Beside these proteins, we recently observed that blood group A antigen was also expressed on some other uncharacterized platelet proteins (70-90 kDa) having electrophoretic mobilities closely resembling those of GPIV and GPV. These findings prompted us further to characterize these latter ABO-expressing platelet proteins. By antigen capture ELISA, wherein the monoclonal antibodies (mAbs) CLB-IVC7 and CLB-SWI6 were used to hold the corresponding antigens GPIV and GPV, human anti-A specifically bound to these proteins derived from A1-platelets; neither GPIV nor GPV derived from A2-, B- or O-platelets bound anti-A. In a Western blot assay using immunoprecipitated GPIV and GPV as antigens, mAb anti-A immunostained GPIV and GPV precipitated from A1, but not from A2 and O platelets. These results conclusively demonstrate that blood group A antigen is expressed on platelet GPIV and GPV.
Authors:
D Stockelberg; M Hou; L Rydberg; J Kutti; H Wadenvik
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Transfusion medicine (Oxford, England)     Volume:  6     ISSN:  0958-7578     ISO Abbreviation:  Transfus Med     Publication Date:  1996 Sep 
Date Detail:
Created Date:  1997-02-19     Completed Date:  1997-02-19     Revised Date:  2008-07-31    
Medline Journal Info:
Nlm Unique ID:  9301182     Medline TA:  Transfus Med     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  243-8     Citation Subset:  IM    
Affiliation:
Department of Medicine, Sahlgrenska University Hospital, University of Göteborg, Sweden.
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MeSH Terms
Descriptor/Qualifier:
ABO Blood-Group System / analysis*
Adult
Animals
Antibodies, Monoclonal / immunology
Antigens, CD36 / chemistry*,  immunology
Antigens, Human Platelet / chemistry*,  immunology
Blood Platelets / chemistry*
Enzyme-Linked Immunosorbent Assay
Female
Glycosylation
Humans
Infant, Newborn
Mice
Platelet Glycoprotein GPIb-IX Complex / chemistry*,  immunology
Protein Processing, Post-Translational
Thrombocytopenia / congenital,  immunology
Chemical
Reg. No./Substance:
0/1a alloantigen, human; 0/ABO Blood-Group System; 0/Antibodies, Monoclonal; 0/Antigens, CD36; 0/Antigens, Human Platelet; 0/Platelet Glycoprotein GPIb-IX Complex

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