Document Detail

Evidence for an expression of blood group A antigen on platelet glycoproteins IV and V.
MedLine Citation:
PMID:  8885154     Owner:  NLM     Status:  MEDLINE    
Blood group ABO antigens are known to be carried by several platelet glycoproteins (GP), e.g. GPIb, GPIIa, GPIIb, GPIIa and PECAM. Beside these proteins, we recently observed that blood group A antigen was also expressed on some other uncharacterized platelet proteins (70-90 kDa) having electrophoretic mobilities closely resembling those of GPIV and GPV. These findings prompted us further to characterize these latter ABO-expressing platelet proteins. By antigen capture ELISA, wherein the monoclonal antibodies (mAbs) CLB-IVC7 and CLB-SWI6 were used to hold the corresponding antigens GPIV and GPV, human anti-A specifically bound to these proteins derived from A1-platelets; neither GPIV nor GPV derived from A2-, B- or O-platelets bound anti-A. In a Western blot assay using immunoprecipitated GPIV and GPV as antigens, mAb anti-A immunostained GPIV and GPV precipitated from A1, but not from A2 and O platelets. These results conclusively demonstrate that blood group A antigen is expressed on platelet GPIV and GPV.
D Stockelberg; M Hou; L Rydberg; J Kutti; H Wadenvik
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Transfusion medicine (Oxford, England)     Volume:  6     ISSN:  0958-7578     ISO Abbreviation:  Transfus Med     Publication Date:  1996 Sep 
Date Detail:
Created Date:  1997-02-19     Completed Date:  1997-02-19     Revised Date:  2008-07-31    
Medline Journal Info:
Nlm Unique ID:  9301182     Medline TA:  Transfus Med     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  243-8     Citation Subset:  IM    
Department of Medicine, Sahlgrenska University Hospital, University of Göteborg, Sweden.
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MeSH Terms
ABO Blood-Group System / analysis*
Antibodies, Monoclonal / immunology
Antigens, CD36 / chemistry*,  immunology
Antigens, Human Platelet / chemistry*,  immunology
Blood Platelets / chemistry*
Enzyme-Linked Immunosorbent Assay
Infant, Newborn
Platelet Glycoprotein GPIb-IX Complex / chemistry*,  immunology
Protein Processing, Post-Translational
Thrombocytopenia / congenital,  immunology
Reg. No./Substance:
0/1a alloantigen, human; 0/ABO Blood-Group System; 0/Antibodies, Monoclonal; 0/Antigens, CD36; 0/Antigens, Human Platelet; 0/Platelet Glycoprotein GPIb-IX Complex

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