| Evidence for the existence of satellite DNA-containing connection between metaphase chromosomes. | |
| | |
MedLine Citation:
|
PMID: 17340617 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Physical connections between mitotic chromosomes have been reported previously. It was assumed that the interchromosome connection was based on the DNA-protein thread. However, the data about DNA sequences and protein component in the thread is fragmentary. We demonstrated on the mouse cultured cell line and prematurely condensed chromosomes that: (a) all four mouse satellite DNA fragments (major and minor satellite, mouse satellite 3 (MS3) and mouse satellite 4 (MS4)) were involved in the thread formation; (b) MS4 was involved in the thread to the least extent among all the other fragments; (c) telomere was never a member of the thread; (d) the thread was synthesized at a late G(2) phase; (e) RNA helicase p68 and CENP-B were among the protein components of the interchromosome connection. It was shown by FACS analysis that in mouse and human cell lines: (1) the flow karyotype spectrums were never free from chromosome aggregates; (2) chromosome association did not depend on the chromosome length and each chromosome was free to associate with the other. |
| | |
Authors:
|
I S Kuznetsova; N I Enukashvily; E M Noniashvili; A N Shatrova; N D Aksenov; V V Zenin; A P Dyban; O I Podgornaya |
Related Documents
:
|
1936957 - Fine mapping of satellite dna sequences along the y chromosome of drosophila melanogast... 3960717 - Sequence heterogeneity within the human alphoid repetitive dna family. 9408737 - Alphoid repetitive dna in human chromosomes. 1840567 - A human chromosome 9-specific alphoid dna repeat spatially resolvable from satellite 3 ... 21722347 - The jigsaw puzzle of our african ancestry: unsolved, or unsolvable? 15972457 - Quantitative trait loci affecting the difference in pigmentation between drosophila yak... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
|
Title: Journal of cellular biochemistry Volume: 101 ISSN: 0730-2312 ISO Abbreviation: J. Cell. Biochem. Publication Date: 2007 Jul |
Date Detail:
|
Created Date: 2007-06-19 Completed Date: 2007-09-11 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 8205768 Medline TA: J Cell Biochem Country: United States |
Other Details:
|
Languages: eng Pagination: 1046-61 Citation Subset: IM |
Affiliation:
|
Institute of Cytology RAS, Tikhoretsky Avenue 4, St Petersburg, Russia. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Cycle / genetics Cell Line Cell Line, Tumor Centromere Protein B / metabolism Chromosomes / genetics*, metabolism DEAD-box RNA Helicases / metabolism DNA, Satellite / genetics*, metabolism Flow Cytometry Humans In Situ Hybridization, Fluorescence Metaphase / genetics* Mice Mitosis / genetics Telomere / genetics, metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Centromere Protein B; 0/DNA, Satellite; EC 3.6.1.-/DEAD-box RNA Helicases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Annexin 1: differential expression in tumor and mast cells in human larynx cancer.
Next Document: Antiproliferative activity of CCN3: involvement of the C-terminal module and post-translational regu...