Document Detail

Evidence for an efflux pump in multidrug-resistant Campylobacter jejuni.
MedLine Citation:
PMID:  8540709     Owner:  NLM     Status:  MEDLINE    
Mechanisms of drug resistance in Campylobacter jejuni were investigated. Mutant strains 34PEFr, which was resistant to pefloxacin (128-fold increase in the MIC), and 34CTXr, which was resistant to cefotaxime (32-fold increase in the MIC) and which was derived from the susceptible parent 34s, were obtained by serial passages on pefloxacin and cefotaxime gradient plates, respectively. Both mutants showed cross-resistance to erythromycin, chloramphenicol, tetracycline, beta-lactams, and quinolones. While the quinolone resistance of strain PEFr could be explained by a mutation at codon 86 of the gyrA gene, the multidrug resistance phenotype of both strains was further investigated. Accumulation of pefloxacin, ciprofloxacin, and minocycline was measured by fluorometry and was found to be lower in the mutant strains than in the parent strain. Preincubation of the cells with carbonyl cyanide m-chlorophenylhydrazone, however, completely abolished this difference. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of outer membrane preparations from both mutant strains showed overexpression of two proteins of 55 and 39 kDa which were absent from the outer membranes of the wild-type strain. These results indicate that in C. jejuni 34PEFr and 34CTXr, multidrug resistance is associated with an efflux system with a broad specificity.
E Charvalos; Y Tselentis; M M Hamzehpour; T Köhler; J C Pechere
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Antimicrobial agents and chemotherapy     Volume:  39     ISSN:  0066-4804     ISO Abbreviation:  Antimicrob. Agents Chemother.     Publication Date:  1995 Sep 
Date Detail:
Created Date:  1996-02-06     Completed Date:  1996-02-06     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0315061     Medline TA:  Antimicrob Agents Chemother     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2019-22     Citation Subset:  IM    
Department of Bacteriology, Parasitology, Zoonoses and Geographical Medicine, School of Health Sciences, University of Crete, Herakleion, Greece.
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MeSH Terms
Anti-Bacterial Agents / metabolism*,  pharmacology
Anti-Infective Agents / metabolism,  pharmacology
Bacterial Outer Membrane Proteins / metabolism
Base Sequence
Campylobacter jejuni / drug effects*,  genetics,  metabolism*
Cefotaxime / metabolism,  pharmacology
Cephalosporins / metabolism,  pharmacology
Drug Resistance, Multiple / physiology*
Molecular Sequence Data
Pefloxacin / metabolism,  pharmacology
Polymerase Chain Reaction
beta-Lactamases / metabolism
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Anti-Infective Agents; 0/Bacterial Outer Membrane Proteins; 0/Cephalosporins; 63527-52-6/Cefotaxime; 70458-92-3/Pefloxacin; EC

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