Document Detail


Evidence for dynamic rearrangements but lack of fate or position restrictions in premigratory avian trunk neural crest.
MedLine Citation:
PMID:  23318636     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Neural crest (NC) cells emerge from the dorsal trunk neural tube (NT) and migrate ventrally to colonize neuronal derivatives, as well as dorsolaterally to form melanocytes. Here, we test whether different dorsoventral levels in the NT have similar or differential ability to contribute to NC cells and their derivatives. To this end, we precisely labeled NT precursors at specific dorsoventral levels of the chick NT using fluorescent dyes and a photoconvertible fluorescent protein. NT and NC cell dynamics were then examined in vivo and in slice culture using two-photon and confocal time-lapse imaging. The results show that NC precursors undergo dynamic rearrangements within the neuroepithelium, yielding an overall ventral to dorsal movement toward the midline of the NT, where they exit in a stochastic manner to populate multiple derivatives. No differences were noted in the ability of precursors from different dorsoventral levels of the NT to contribute to NC derivatives, with the exception of sympathetic ganglia, which appeared to be 'filled' by the first population to emigrate. Rather than restricted developmental potential, however, this is probably due to a matter of timing.
Authors:
Mary C McKinney; Kazumi Fukatsu; Jason Morrison; Rebecca McLennan; Marianne E Bronner; Paul M Kulesa
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2013-01-14
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  140     ISSN:  1477-9129     ISO Abbreviation:  Development     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-30     Completed Date:  2013-03-26     Revised Date:  2014-02-18    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  820-30     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Movement / physiology*
Chick Embryo
Computational Biology
Embryonic Stem Cells / cytology*,  metabolism
Fluorescent Dyes
Microscopy, Confocal
Neural Crest / embryology*
Neural Tube / cytology*,  embryology
Neuroepithelial Cells / physiology*
Reverse Transcriptase Polymerase Chain Reaction
Time Factors
Time-Lapse Imaging
Grant Support
ID/Acronym/Agency:
1R01HD057922/HD/NICHD NIH HHS; DE16459/DE/NIDCR NIH HHS; HD037105/HD/NICHD NIH HHS; P30-HD18655/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Fluorescent Dyes
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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