| Evidence for dual regulation by protein kinases A and C of thyrotropin-releasing hormone receptor mRNA in GH3 cells. | |
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MedLine Citation:
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PMID: 1659532 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We showed previously that TRH down-regulates TRH receptor (TRH-R) mRNA in GH3 cells by a mechanism that appears to be mediated by protein kinase C. Here we show that vasoactive intestinal peptide (VIP) down-regulates TRH-R mRNA and present evidence that this action is mediated by protein kinase A. In GH3 cells, VIP caused a time- and concentration-dependent decrease in TRH-R mRNA. This VIP effect was simulated by 8-(4-chlorophenylthio)-cAMP, forskolin, cholera toxin and 1-methyl-3-isobutylxanthine. When cells were incubated with agents that elevate cAMP and TRH or phorbol 12-myristate 13-acetate, the decrease in TRH-R mRNA was greater than with either agent alone. When cells were pre-incubated with H-7 [1-(5-isoquinolinesulfonyl)-2-methylpiperazine dihydrochloride], an inhibitor of protein kinases, the effects of VIP, TRH and phorbol 12-myristate 13-acetate were inhibited. We suggest that VIP, via protein kinase A, and TRH, via protein kinase C, dually regulate TRH-R mRNA. |
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Authors:
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J Fujimoto; M C Gershengorn |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Endocrinology Volume: 129 ISSN: 0013-7227 ISO Abbreviation: Endocrinology Publication Date: 1991 Dec |
Date Detail:
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Created Date: 1991-12-27 Completed Date: 1991-12-27 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 3430-2 Citation Subset: AIM; IM |
Affiliation:
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Department of Medicine, Cornell University Medical College, New York Hospital, NY 10021. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line Cyclic AMP / physiology Enzyme Activation Gene Expression Regulation / drug effects* Protein Kinase C / antagonists & inhibitors, metabolism* Protein Kinase Inhibitors Protein Kinases / metabolism* RNA, Messenger / metabolism* Rats Receptors, Neurotransmitter / genetics* Receptors, Thyrotropin-Releasing Hormone Thyrotropin-Releasing Hormone / pharmacology Vasoactive Intestinal Peptide / pharmacology* |
| Grant Support | |
ID/Acronym/Agency:
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DK43036/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Protein Kinase Inhibitors; 0/RNA, Messenger; 0/Receptors, Neurotransmitter; 0/Receptors, Thyrotropin-Releasing Hormone; 24305-27-9/Thyrotropin-Releasing Hormone; 37221-79-7/Vasoactive Intestinal Peptide; 60-92-4/Cyclic AMP; EC 2.7.-/Protein Kinases; EC 2.7.11.13/Protein Kinase C |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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