| Evidence for distinct isoenzymic forms of a cell surface protease on normal colonic cells and on carcinoma cells from the same colon. | |
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MedLine Citation:
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PMID: 1708219 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Both normal and carcinoma cells of the colon possess the cell surface protease guanidinobenzoatase (GB). GB is similar to plasminogen activator. In fixed sections, the insolubilised GB can be located by the fluorescent probe 9-amino acridine (9-AA) which binds to the active centre of GB, causing cells possessing active GB to fluoresce yellow. Both cell types possess cytoplasmic proteins which can be extracted in isotonic saline and were shown to inhibit their respective cell surface GB. The formation of an enzyme-inhibitor complex was demonstrated by the failure of 9-AA to react with the cell surface GB inhibitor complex. The data presented indicate that the colonic carcinoma cells possess an isoenzymic form of GB not recognised by the inhibitors of GB extracted from normal colonic cells. |
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Authors:
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F S Steven; M M Griffin; I C Talbot |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Anticancer research Volume: 11 ISSN: 0250-7005 ISO Abbreviation: Anticancer Res. Publication Date: 1991 Jan-Feb |
Date Detail:
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Created Date: 1991-05-23 Completed Date: 1991-05-23 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8102988 Medline TA: Anticancer Res Country: GREECE |
Other Details:
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Languages: eng Pagination: 143-50 Citation Subset: IM |
Affiliation:
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Department of Biochemistry and Molecular Biology, School of Biological Sciences, University of Manchester, U.K. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aminacrine Carboxylic Ester Hydrolases / analysis* Colon / cytology, enzymology*, pathology Colonic Neoplasms / enzymology*, pathology Endopeptidases* Fluorescent Dyes Humans Intestinal Mucosa / cytology, enzymology*, pathology Isoenzymes / analysis* Reference Values Staining and Labeling Tumor Markers, Biological / analysis* |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 0/Isoenzymes; 0/Tumor Markers, Biological; 90-45-9/Aminacrine; EC 3.1.1.-/Carboxylic Ester Hydrolases; EC 3.1.1.-/guanidinobenzoate esterase; EC 3.4.-/Endopeptidases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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