Document Detail

Evidence for distinct isoenzymic forms of a cell surface protease on normal colonic cells and on carcinoma cells from the same colon.
MedLine Citation:
PMID:  1708219     Owner:  NLM     Status:  MEDLINE    
Both normal and carcinoma cells of the colon possess the cell surface protease guanidinobenzoatase (GB). GB is similar to plasminogen activator. In fixed sections, the insolubilised GB can be located by the fluorescent probe 9-amino acridine (9-AA) which binds to the active centre of GB, causing cells possessing active GB to fluoresce yellow. Both cell types possess cytoplasmic proteins which can be extracted in isotonic saline and were shown to inhibit their respective cell surface GB. The formation of an enzyme-inhibitor complex was demonstrated by the failure of 9-AA to react with the cell surface GB inhibitor complex. The data presented indicate that the colonic carcinoma cells possess an isoenzymic form of GB not recognised by the inhibitors of GB extracted from normal colonic cells.
F S Steven; M M Griffin; I C Talbot
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Anticancer research     Volume:  11     ISSN:  0250-7005     ISO Abbreviation:  Anticancer Res.     Publication Date:    1991 Jan-Feb
Date Detail:
Created Date:  1991-05-23     Completed Date:  1991-05-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  GREECE    
Other Details:
Languages:  eng     Pagination:  143-50     Citation Subset:  IM    
Department of Biochemistry and Molecular Biology, School of Biological Sciences, University of Manchester, U.K.
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MeSH Terms
Carboxylic Ester Hydrolases / analysis*
Colon / cytology,  enzymology*,  pathology
Colonic Neoplasms / enzymology*,  pathology
Fluorescent Dyes
Intestinal Mucosa / cytology,  enzymology*,  pathology
Isoenzymes / analysis*
Reference Values
Staining and Labeling
Tumor Markers, Biological / analysis*
Reg. No./Substance:
0/Fluorescent Dyes; 0/Isoenzymes; 0/Tumor Markers, Biological; 90-45-9/Aminacrine; EC 3.1.1.-/Carboxylic Ester Hydrolases; EC 3.1.1.-/guanidinobenzoate esterase; EC 3.4.-/Endopeptidases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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