| Evidence for discontinuous replication of circular mitochondrial DNA molecules from Novikoff rat ascites hepatoma cells. | |
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MedLine Citation:
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PMID: 4362136 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Double-forked circular molecules of mitochondrial DNA (mtDNA) from rat tissues, indicated by their form and size to be replicative intermediates, are of two structurally distinct classes. Molecules of the first class are totally double stranded. Molecules of the second class are defined by one daughter segment being totally or partially single stranded. Length histograms of daughter segments measuring between 2% and 44% of the total 5-microm molecular contour were constructed from samples of both classes of replicating molecules derived from mtDNA or Novikoff rat ascites hepatoma cells. For single strand-containing molecules, the lengths fell into eight distinct, reproducible groups with mean values separated by 4.1-7.6% of the circular contour length. For totally double stranded molecules, the lengths fell into seven groups, corresponding to seven of the groups found for single strand-containing molecules. These results suggest that along at least 44% of the contour of mtDNA molecules there exist discrete points at which DNA synthesis tends to be arrested. This may indicate that there are pauses in normal mtDNA synthesis. However, as the DNA used in these experiments was isolated from mitochondrial fractions, the findings may indicate that continuation of synthesis beyond specific points on the nucleotide strands requires a factor which is not available after cell disruption. |
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Authors:
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K Koike; D R Wolstenholme |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of cell biology Volume: 61 ISSN: 0021-9525 ISO Abbreviation: J. Cell Biol. Publication Date: 1974 Apr |
Date Detail:
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Created Date: 1974-05-29 Completed Date: 1974-05-29 Revised Date: 2010-06-22 |
Medline Journal Info:
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Nlm Unique ID: 0375356 Medline TA: J Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 14-25 Citation Subset: IM |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carcinoma, Hepatocellular / metabolism* Cell Line DNA Replication* DNA, Circular / biosynthesis DNA, Mitochondrial / biosynthesis DNA, Neoplasm / biosynthesis DNA, Single-Stranded / biosynthesis Liver Neoplasms / metabolism Microscopy, Electron Neoplasms, Experimental / metabolism Rats |
| Chemical | |
Reg. No./Substance:
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0/DNA, Circular; 0/DNA, Mitochondrial; 0/DNA, Neoplasm; 0/DNA, Single-Stranded |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Cell Biol ISSN: 0021-9525 ISSN: 1540-8140 Publisher: The Rockefeller University Press |
Article Information Download PDF  Copyright © 1974 by The Rockefeller University Press Received Day: 13 Month: 8 Year: 1973 Revision Received Day: 12 Month: 11 Year: 1973 Print publication date: Day: 1 Month: 4 Year: 1974 Volume: 61 Issue: 1 First Page: 14 Last Page: 25 ID: 2109274 PubMed Id: 4362136 |
| EVIDENCE FOR DISCONTINUOUS REPLICATION OF CIRCULAR MITOCHONDRIAL DNA MOLECULES FROM NOVIKOFF RAT ASCITES HEPATOMA CELLS | |
| Katsuro Koike | |
| David R. Wolstenholme | |
| From the Division of Biology, Kansas State University, Manhattan, Kansas 66502 and the Department of Biology, University of Utah, Salt Lake City, Utah 84112. |
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| Dr. Koike's present address is the Cancer Institute (Japanese Foundation for Cancer Research), Tokyo 170, Japan. Dr. Wolstenholme's present address is the Department of Biology, University of Utah, Salt Lake City, Utah 84112. |
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