| Evidence for a dimeric structure of rat liver serine dehydratase. | |
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MedLine Citation:
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PMID: 11906824 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Rat liver serine dehydratase (SDH) is known to be involved in gluconeogenesis. It has long been believed to be a dimeric protein with the subunit molecular weight (M(r)) of 34,000. Recently, sheep liver SDH was reported to be a monomer with a M(r) of 38,000. The native M(r) of rat SDH was only determined by the ultracentrifugation method more than three decades ago, and that of sheep SDH was done by the method of gel chromatography. The primary to quaternary structures of a given enzyme in a specific mammalian organ are usually conserved among various species. The aim of the present investigation is to clarify the structural differences between rat and sheep SDHs. First, we found that the amino acid composition reported for sheep SDH was statistically similar to that of rat SDH. Second, immunoblot analysis using anti-rat SDH IgG as the probe showed the size of sheep SDH to be a M(r) of 30,500, whereas that of SDH was about M(r) of 35,000. On the other hand, the native size of rat SDH was assessed by two methods: (1) the laser light scattering method demonstrated that rat SDH had a M(r) of 66,800, consistent with the previous value (M(r)=64,000); (2) cross-linking experiments of the purified rat SDH with dimethyl suberimidate revealed the existence of a dimeric form by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The present results clearly confirm that rat SDH is a dimer, and suggest that sheep SDH is similar to rat SDH immunologically, but with a molecular weight 7500 smaller than reported previously. |
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Authors:
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Hirofumi Ogawa; Tomoharu Gomi; Fusao Takusagawa; Toru Masuda; Takuya Goto; Takanobu Kan; Num-Ho Huh |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The international journal of biochemistry & cell biology Volume: 34 ISSN: 1357-2725 ISO Abbreviation: Int. J. Biochem. Cell Biol. Publication Date: 2002 May |
Date Detail:
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Created Date: 2002-03-21 Completed Date: 2002-06-28 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9508482 Medline TA: Int J Biochem Cell Biol Country: England |
Other Details:
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Languages: eng Pagination: 533-43 Citation Subset: IM |
Affiliation:
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Faculty of Medicine, Department of Biochemistry, Toyama Medical and Pharmaceutical University, Toyama 930-0194, Japan. hogawa@ms.toyama-mpu.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis Animals Chromatography, Gel Cross-Linking Reagents / chemistry Dimerization Dimethyl Suberimidate / chemistry Humans L-Serine Dehydratase / chemistry*, isolation & purification, metabolism Lasers Liver / enzymology* Male Molecular Weight Protein Structure, Quaternary Rats Rats, Sprague-Dawley Sheep |
| Grant Support | |
ID/Acronym/Agency:
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GM37233/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Cross-Linking Reagents; 29878-26-0/Dimethyl Suberimidate; EC 4.3.1.17/L-Serine Dehydratase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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