| Evidence of the cross talk between Wnt and Notch signaling pathways in non-small-cell lung cancer (NSCLC): Notch3-siRNA weakens the effect of LiCl on the cell cycle of NSCLC cell lines. | |
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MedLine Citation:
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PMID: 20614134 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Aberrant activations of Wnt and Notch signaling pathways are individually reported to be associated with the pathogenesis of non-small-cell lung cancer (NSCLC). However, the data about the cross talk between the two signaling pathways are still limited. To elucidate potential Wnt/Notch cross talk within NSCLC, we examined the impact of Notch3 activity on LiCl-induced cell cycle changes. METHODS: The lung cancer cell lines were treated with LiCl, a Wnt activator, in the absence or presence of Notch3-siRNA. Cell cycles and the expression of the regulators of cell cycle, c-MYC, p21 and Skp2 (S phase kinase-associated protein 2) were measured after treatment. RESULTS: The treatment with LiCl increased the percent of cells at S phase and G phase and the expression of c-MYC and Skp2 and decreased the expression of p21. Moreover, the expression of Notch3 and its down-stream genes, HES-1 and HEYL, was up-regulated by LiCl. Notch3-siRNA weakened the effect of LiCl on the cell cycle and resulted in attenuation of the LiCl-induced increment of c-MYC and Skp2 and the LiCl-induced decrement of p21. CONCLUSIONS: These data suggest that Notch3 activation cooperatively takes part in the LiCl-induced cell cycle changes, at least partially, associated with c-MYC, Skp2 and p21. |
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Authors:
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Chunyan Li; Ying Zhang; Yao Lu; Zeshi Cui; Miao Yu; Siyang Zhang; Xiaoxia Xue |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-08 |
Journal Detail:
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Title: Journal of cancer research and clinical oncology Volume: 137 ISSN: 1432-1335 ISO Abbreviation: J. Cancer Res. Clin. Oncol. Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-04-12 Completed Date: 2011-06-02 Revised Date: 2011-11-02 |
Medline Journal Info:
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Nlm Unique ID: 7902060 Medline TA: J Cancer Res Clin Oncol Country: Germany |
Other Details:
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Languages: eng Pagination: 771-8 Citation Subset: IM |
Affiliation:
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Center of Laboratory Technology and Experimental Medicine, China Medical University, 110001, Shenyang, People's Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Carcinoma, Non-Small-Cell Lung
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pathology* Cell Cycle / drug effects Cell Line, Tumor Cyclin-Dependent Kinase Inhibitor p21 / analysis Glycogen Synthase Kinase 3 / physiology Humans Lithium Chloride / pharmacology* Proto-Oncogene Proteins c-myc / analysis RNA, Small Interfering / genetics* Receptors, Notch / antagonists & inhibitors, physiology* S-Phase Kinase-Associated Proteins / analysis Signal Transduction / physiology* Wnt Proteins / physiology* |
| Chemical | |
Reg. No./Substance:
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0/CDKN1A protein, human; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/MYC protein, human; 0/NOTCH3 protein, human; 0/Proto-Oncogene Proteins c-myc; 0/RNA, Small Interfering; 0/Receptors, Notch; 0/S-Phase Kinase-Associated Proteins; 0/Wnt Proteins; 7447-41-8/Lithium Chloride; EC 2.7.11.1/glycogen synthase kinase 3 beta; EC 2.7.11.26/Glycogen Synthase Kinase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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