Document Detail


Evidence for cross-bridge order in contraction of glycerinated skeletal muscle.
MedLine Citation:
PMID:  6584869     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The linear dichroism of iodoacetylrhodamine labels attached to the single reactive thiol groups of myosin heads was measured to determine the spatial orientation of myosin cross-bridges in single glycerinated skeletal muscle fibers. We have shown previously that in rigor the chromophoric labels are well ordered and assume an orientation nearly perpendicular to the fiber axis; in the presence of MgADP, a large fraction of probe remains well ordered but the probe attitude assumes a more slanted orientation; in relaxed muscle, the probe order is largely lost, implying a high degree of cross-bridge disorder. In this paper, we report that during isometric contraction a large fraction of the probe shows a high degree of order, suggesting the attachment of approximately equal to 65% of the cross-bridges to actin. These ordered cross-bridges have a probe attitude similar to that of the MgADP-induced static state and hence are in a mechanical state quite distinct from rigor.
Authors:
T P Burghardt; T Ando; J Borejdo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  80     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1983 Dec 
Date Detail:
Created Date:  1984-05-11     Completed Date:  1984-05-11     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7515-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenosine Diphosphate / pharmacology
Animals
Glycerol / pharmacology*
Magnesium / pharmacology
Mathematics
Models, Biological
Muscle Contraction / drug effects*
Muscles / physiology*
Myosins / metabolism
Rabbits
Grant Support
ID/Acronym/Agency:
HL-16683/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
56-81-5/Glycerol; 58-64-0/Adenosine Diphosphate; 7439-95-4/Magnesium; EC 3.6.4.1/Myosins
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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