Document Detail


Evidence for covalent linkage between xyloglucan and acidic pectins in suspension-cultured rose cells.
MedLine Citation:
PMID:  10945222     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Neutral xyloglucan was purified from the cell walls of suspension-cultured rose (Rosa sp. 'Paul's Scarlet') cells by alkali extraction, ethanol precipitation and anion-exchange chromatography on 'Q-Sepharose FastFlow'. The procedure recovered 70% of the total xyloglucan at about 95% purity in the neutral fraction. The remaining 30% of the xyloglucan was anionic, as demonstrated both by anion-exchange chromatography at pH 4.7 and by high-voltage electrophoresis at pH 6.5. Alkali did not cause neutral xyloglucan to become anionic, indicating that the anionic nature of the rose xyloglucan was not an artefact of the extraction procedure. Pre-incubation of neutral [3H]xyloglucan with any of ten non-radioactive acidic polysaccharides did not cause the radioactive material to become anionic as judged by electrophoresis, indicating that stable complexes between neutral xyloglucan and acidic polysaccharides were not readily formed in vitro. The anionic xyloglucan did not lose its charge in the presence of 8 M urea or after a second treatment with NaOH, indicating that its anionic nature was not due to hydrogen-bonding of xyloglucan to an acidic polymer. Proteinase did not affect the anionic xyloglucan, indicating that it was not associated with an acidic protein. Cellulase converted the anionic xyloglucan to the expected neutral nonasaccharide and heptasaccharide, indicating that the repeatunits of the xyloglucan did not contain acidic residues. Endo-polygalacturonase converted about 40% of the anionic xyloglucan to neutral material. Arabinanase and galactanase also converted appreciable proportions of the anionic xyloglucan to neutral material. These results show that about 30% of the xyloglucan in the cell walls of suspension-cultured rose cells exists in covalently-linked complexes with acidic pectins.
Authors:
J E Thompson; S C Fry
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Planta     Volume:  211     ISSN:  0032-0935     ISO Abbreviation:  Planta     Publication Date:  2000 Jul 
Date Detail:
Created Date:  2000-11-13     Completed Date:  2001-01-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1250576     Medline TA:  Planta     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  275-86     Citation Subset:  IM    
Affiliation:
The Edinburgh Cell Wall Group, Institute of Cell and Molecular Biology, The University of Edinburgh, UK.
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MeSH Terms
Descriptor/Qualifier:
Arabinose / metabolism
Artifacts
Cell Wall / physiology
Cells, Cultured
Cellulase
Cellulose / metabolism
Chromatography, Ion Exchange
Electrophoresis, Paper
Endopeptidases
Glucans*
Oligosaccharides / chemistry,  isolation & purification
Pectins / chemistry,  isolation & purification,  metabolism*
Polygalacturonase
Polysaccharides / chemistry,  isolation & purification,  metabolism*
Radioisotope Dilution Technique
Rosales / cytology*,  physiology
Tritium
Xylans*
Chemical
Reg. No./Substance:
0/Glucans; 0/Oligosaccharides; 0/Pectins; 0/Polysaccharides; 0/Xylans; 10028-17-8/Tritium; 147-81-9/Arabinose; 37294-28-3/xyloglucan; 9000-69-5/pectin; 9004-34-6/Cellulose; EC 3.2.1.15/Polygalacturonase; EC 3.2.1.4/Cellulase; EC 3.4.-/Endopeptidases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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