Document Detail


Evidence for compartmentation of uridine nucleotide pools in rat hepatoma cells.
MedLine Citation:
PMID:  570061     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Interaction between the de novo and salvage pathways of pyrimidine metabolism was studied in a line of rat hepatoma cells by co-labelling with [14C]-uridine and [3H]orotate. A difference in the ratio of 14C/3H between CTP and UTP in acid-soluble nucleotide pool was reflected in the corresponding ratios in CMP and UMP in RNA, with uridine labelling cytidine nucleotides relatively more effectively than orotate. These results are not compatible with the concept of a single UTP pool, and a new model for pyrimidine anabolic pathways, based on compartmentation of de novo from salvage pathways, is proposed.
Authors:
M J Losman; E H Harley
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  521     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1978 Dec 
Date Detail:
Created Date:  1979-04-25     Completed Date:  1979-04-25     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  762-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Cytidine Monophosphate / metabolism
Liver Neoplasms, Experimental / metabolism*
Orotic Acid / metabolism
RNA, Neoplasm / metabolism
Uracil Nucleotides / metabolism*
Uridine Monophosphate / metabolism
Chemical
Reg. No./Substance:
0/RNA, Neoplasm; 0/Uracil Nucleotides; 58-97-9/Uridine Monophosphate; 63-37-6/Cytidine Monophosphate; 65-86-1/Orotic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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