Document Detail

Evidence for a compartmentalized B cell response as characterized by IgG epitope specificity in human ocular toxoplasmosis.
MedLine Citation:
PMID:  11714789     Owner:  NLM     Status:  MEDLINE    
Infectious agents in the eye induce both a local and a systemic humoral immune response. Previously, differences in Ag recognition were observed between systemic and ocular derived IgG of patients with ocular toxoplasmosis. This finding implied a nonrandom distribution of IgG-producing B cells in the inflamed eye. In the present study, we compared the intraocular and systemic B cell responses of patients with ocular toxoplasmosis to a single Toxoplasma gondii Ag. Two series of C-terminally deleted recombinant T. gondii GRA-2 proteins were constructed to delineate IgG B cell epitopes of paired ocular and serum samples. Differences in epitope region recognition between the ocular and systemic compartment were detected in 9 of 13 patients. The difference in distribution of GRA-2 epitopes between paired samples is indicative of a local GRA-2 specific B cell population functionally different from the systemic GRA-2-specific B cell population. Our results suggest a selective activation of a subset of B cells locally in nonlymphoid tissue.
V N Klaren; R Peek
Related Documents :
15304549 - Biological activity of the g-quadruplex ligand rhps4 (3,11-difluoro-6,8,13-trimethyl-8h...
17587609 - Replicative senescence, telomere shortening and cell proliferation rate in gaddi goat's...
17697939 - Partial uncoupling of oxidative phosphorylation induces premature senescence in human f...
12820429 - Changes in telomerase activity, expression and splicing in response to differentiation ...
4021559 - Membrane glycoprotein changes during the senescence of normal human diploid fibroblasts...
15479119 - Spontaneous cell transformation: karyoplasts derived from multinucleated cells produce ...
24224529 - Quantitative proteomics reveals significant changes in cell shape and an energy shift a...
11283949 - Structural abnormalities develop in the brain after ablation of the gene encoding nonmu...
2776519 - The establishment of regional identity in the xenopus blastula.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  167     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  2001 Dec 
Date Detail:
Created Date:  2001-11-20     Completed Date:  2002-01-02     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6263-9     Citation Subset:  AIM; IM    
Department of Molecular Immunology, The Netherlands Ophthalmic Research Institute, Amsterdam, The Netherlands.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Antibody Specificity*
Antigen-Antibody Reactions
Antigens, Protozoan / analysis,  immunology
B-Lymphocyte Subsets / immunology*,  metabolism
B-Lymphocytes / immunology*,  metabolism
Epitope Mapping / methods
Epitopes, B-Lymphocyte / analysis,  immunology*
Immunodominant Epitopes / analysis,  immunology
Immunoglobulin G / biosynthesis*
Middle Aged
Peptide Fragments / analysis,  immunology
Protozoan Proteins / analysis,  immunology
Toxoplasmosis, Ocular / immunology*
Reg. No./Substance:
0/Antigens, Protozoan; 0/Epitopes, B-Lymphocyte; 0/Gra2 protein, Toxoplasma gondii; 0/Immunodominant Epitopes; 0/Immunoglobulin G; 0/Peptide Fragments; 0/Protozoan Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Cre recombinase-mediated inactivation of H-2Dd transgene expression: evidence for partial missing se...
Next Document:  Requirement for Ca2+/calmodulin-dependent kinase type IV/Gr in setting the thymocyte selection thres...