Document Detail


Evidence for a compartmentalized B cell response as characterized by IgG epitope specificity in human ocular toxoplasmosis.
MedLine Citation:
PMID:  11714789     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Infectious agents in the eye induce both a local and a systemic humoral immune response. Previously, differences in Ag recognition were observed between systemic and ocular derived IgG of patients with ocular toxoplasmosis. This finding implied a nonrandom distribution of IgG-producing B cells in the inflamed eye. In the present study, we compared the intraocular and systemic B cell responses of patients with ocular toxoplasmosis to a single Toxoplasma gondii Ag. Two series of C-terminally deleted recombinant T. gondii GRA-2 proteins were constructed to delineate IgG B cell epitopes of paired ocular and serum samples. Differences in epitope region recognition between the ocular and systemic compartment were detected in 9 of 13 patients. The difference in distribution of GRA-2 epitopes between paired samples is indicative of a local GRA-2 specific B cell population functionally different from the systemic GRA-2-specific B cell population. Our results suggest a selective activation of a subset of B cells locally in nonlymphoid tissue.
Authors:
V N Klaren; R Peek
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  167     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  2001 Dec 
Date Detail:
Created Date:  2001-11-20     Completed Date:  2002-01-02     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6263-9     Citation Subset:  AIM; IM    
Affiliation:
Department of Molecular Immunology, The Netherlands Ophthalmic Research Institute, Amsterdam, The Netherlands. v.klaren@ioi.knaw.nl
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Antibody Specificity*
Antigen-Antibody Reactions
Antigens, Protozoan / analysis,  immunology
B-Lymphocyte Subsets / immunology*,  metabolism
B-Lymphocytes / immunology*,  metabolism
Epitope Mapping / methods
Epitopes, B-Lymphocyte / analysis,  immunology*
Female
Humans
Immunodominant Epitopes / analysis,  immunology
Immunoglobulin G / biosynthesis*
Male
Middle Aged
Peptide Fragments / analysis,  immunology
Protozoan Proteins / analysis,  immunology
Toxoplasmosis, Ocular / immunology*
Chemical
Reg. No./Substance:
0/Antigens, Protozoan; 0/Epitopes, B-Lymphocyte; 0/Gra2 protein, Toxoplasma gondii; 0/Immunodominant Epitopes; 0/Immunoglobulin G; 0/Peptide Fragments; 0/Protozoan Proteins

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