Document Detail


Evidence for cell apoptosis suppressing white spot syndrome virus replication in Procambarus clarkii at high temperature.
MedLine Citation:
PMID:  23209074     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
In shrimp, higher water temperatures (~32°C) can suppress the ability of white spot syndrome virus (WSSV) to replicate and cause mortality, but the mechanisms remain unclear. To investigate whether cell apoptosis might be involved, a Tdt-mediated dUTP nick-end label (TUNEL) method was used to assess levels of chromosomal DNA fragmentation in hepatopancreas and gill cells of Procambarus clarkii crayfish infected with WSSV and maintained at either 32 ± 1°C or 24 ± 1°C. Based on relative cell numbers with yellow-green colored TUNEL-positive nuclei, the apoptotic index was elevated in WSSV-infected crayfish maintained at 32°C. In gill tissue sections examined by transmission electron microscope, cells with nuclei displaying apoptotic bodies or marginated, condensed and fragmented chromatin without concurrent cell cytoplasm damage were also more prevalent. Flow cytometry sorting of annexin-stained cells showed apoptosis to be most prevalent in granular haemocytes, and assays for caspase-3 activity showed it to be most elevated in hepatopancreas tissue. Despite these indicators of cell apoptosis but consistent with WSSV replication being restricted at elevated temperatures, no increases in transcription of the viral anti-apoptosis genes ORF390 and ORF222 were detected by RT-PCR in shrimp maintained at 32°C, possibly due to the elevated levels of cellular apoptosis.
Authors:
Xiao-Guo Wu; Hai-Tao Xiong; Yi-Zhen Wang; Hua-Hua Du
Related Documents :
22955924 - Endocytosis of soluble immune complexes leads to their clearance by fcγriiib but induc...
24372204 - A lipid anchor improves the protective effect of ectoine in inflammation.
24198734 - Arzanol, a potent mpges-1 inhibitor: novel anti-inflammatory agent.
24717224 - The role of soluble fibrinogen-like protein 2 in transplantation: protection or damage.
15728244 - The expression and roles of toll-like receptors in the biology of the human neutrophil.
19127214 - Interleukin-10 versus dexamethasone: effects on polymorphonuclear leukocyte functions o...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Diseases of aquatic organisms     Volume:  102     ISSN:  0177-5103     ISO Abbreviation:  Dis. Aquat. Org.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-04     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8807037     Medline TA:  Dis Aquat Organ     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  13-21     Citation Subset:  IM    
Affiliation:
Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Feed Science Institute, Zhejiang University, Hangzhou 310029, PR China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Cellular kinetics in growth anomalies of the scleractinian corals Porites australiensis and Montipor...
Next Document:  Cohort study of effect of vaccination on pancreas disease in Norwegian salmon aquaculture.