Document Detail

Evidence for a blockwise distribution of acetyl groups onto homogalacturonans from a commercial sugar beet (Beta vulgaris) pectin.
MedLine Citation:
PMID:  18448141     Owner:  NLM     Status:  MEDLINE    
Commercial acid-extracted sugar beet pectin was extensively hydrolysed using an endo-polygalacturonase (AnPGI from Aspergillus niger or AnPGII from A. niger or FmPG from Fusarium moniliforme) in combination with Aspergillus aculeatus pectin methyl-esterase (AaPME). The homogalacturonan-derived oligogalacturonates released were quantified by high-performance anion-exchange chromatography and their structure determined by mass spectrometry. The different endo-polygalacturonases exhibited variable tolerance towards acetyl groups. AnPGI was the most active and FmPG the less. A hypothetical homogalacturonan was constructed using the AnPGI-recovered oligogalacturonates as building blocks and the validity of the model was checked taking into account FmPG observed requirements and hydrolysis products. A blockwise repartition of the acetyl groups onto sugar beet pectin homogalacturonan is proposed.
Marie-Christine Ralet; Marie-Jeanne Crépeau; Estelle Bonnin
Publication Detail:
Type:  Journal Article     Date:  2008-04-28
Journal Detail:
Title:  Phytochemistry     Volume:  69     ISSN:  0031-9422     ISO Abbreviation:  Phytochemistry     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-06-05     Completed Date:  2008-09-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0151434     Medline TA:  Phytochemistry     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1903-9     Citation Subset:  IM    
INRA, UR1268 Biopolymères Interactions Assemblage, Rue de la Géraudière, B.P. 71627, F-44300 Nantes, France.
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MeSH Terms
Beta vulgaris / chemistry*
Carbohydrate Conformation
Carbohydrate Sequence
Chromatography, Ion Exchange
Galactose / chemistry*
Pectins / chemistry*
Reg. No./Substance:
0/Pectins; 26566-61-0/Galactose; 9000-69-5/pectin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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