| Evidence for a blockwise distribution of acetyl groups onto homogalacturonans from a commercial sugar beet (Beta vulgaris) pectin. | |
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MedLine Citation:
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PMID: 18448141 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Commercial acid-extracted sugar beet pectin was extensively hydrolysed using an endo-polygalacturonase (AnPGI from Aspergillus niger or AnPGII from A. niger or FmPG from Fusarium moniliforme) in combination with Aspergillus aculeatus pectin methyl-esterase (AaPME). The homogalacturonan-derived oligogalacturonates released were quantified by high-performance anion-exchange chromatography and their structure determined by mass spectrometry. The different endo-polygalacturonases exhibited variable tolerance towards acetyl groups. AnPGI was the most active and FmPG the less. A hypothetical homogalacturonan was constructed using the AnPGI-recovered oligogalacturonates as building blocks and the validity of the model was checked taking into account FmPG observed requirements and hydrolysis products. A blockwise repartition of the acetyl groups onto sugar beet pectin homogalacturonan is proposed. |
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Authors:
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Marie-Christine Ralet; Marie-Jeanne Crépeau; Estelle Bonnin |
Publication Detail:
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Type: Journal Article Date: 2008-04-28 |
Journal Detail:
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Title: Phytochemistry Volume: 69 ISSN: 0031-9422 ISO Abbreviation: Phytochemistry Publication Date: 2008 Jun |
Date Detail:
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Created Date: 2008-06-05 Completed Date: 2008-09-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0151434 Medline TA: Phytochemistry Country: United States |
Other Details:
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Languages: eng Pagination: 1903-9 Citation Subset: IM |
Affiliation:
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INRA, UR1268 Biopolymères Interactions Assemblage, Rue de la Géraudière, B.P. 71627, F-44300 Nantes, France. ralet@nantes.inra.fr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetylation Beta vulgaris / chemistry* Carbohydrate Conformation Carbohydrate Sequence Chromatography, Ion Exchange Galactose / chemistry* Hydrolysis Methylation Pectins / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Pectins; 26566-61-0/Galactose; 9000-69-5/pectin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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