Document Detail

Evidence for a biologically active, carrier-bound form of non-suppressible insulin-like activity (NSILA) in human serum.
MedLine Citation:
PMID:  6852354     Owner:  NLM     Status:  MEDLINE    
1. Quantitative gel filtration of Cohn fraction IV-1 has shown that high MW NSILA recovered under "neutral" conditions (pH 5.5) was greater in magnitude (approximately X 2) than acid-stable high MW NSILA obtained under acid conditions. 2. Recombination, at neutral poH, of high and low MW NSILA, obtained by previous acid gel filtration, gave quantitative recovery of activity which was solely of high MW. 3. These data indicate that a biologically active, and dissociable high MW form of NSILA exists in serum. 4. Con A-Sepharose affinity chromatography gave one unbound NSILA fraction and two bound fractions, which both gave high and low MW activity after acid chromatography. 5. This study provides the first evidence for a carrier-bound form of low MW NSILA which retains biological activity.
H J Cornell; A C Herington
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The International journal of biochemistry     Volume:  15     ISSN:  0020-711X     ISO Abbreviation:  Int. J. Biochem.     Publication Date:  1983  
Date Detail:
Created Date:  1983-07-29     Completed Date:  1983-07-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0250365     Medline TA:  Int J Biochem     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  553-8     Citation Subset:  IM    
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MeSH Terms
Chromatography, Affinity
Chromatography, Gel
Hydrogen-Ion Concentration
Molecular Weight
Nonsuppressible Insulin-Like Activity / isolation & purification*
Sepharose / analogs & derivatives
Reg. No./Substance:
0/Nonsuppressible Insulin-Like Activity; 0/concanavalin A-sepharose; 9012-36-6/Sepharose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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