Document Detail


Evidence for a bidirectional prostaglandin endoperoxide shunt between platelets and the bovine coronary artery.
MedLine Citation:
PMID:  2493809     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
While platelets have been shown to be capable of supplying prostaglandin (PG) H2 to endothelial cells in culture for PGI2 synthesis, endothelial cells have been shown unable to supply PGH2 to platelets for thromboxane (TX) A2 synthesis. We incubated rings of the bovine coronary artery (BCAR) with human platelets treated with aspirin (to inhibit cyclooxygenase) or CGS 13080 (to inhibit TXA2 synthase) in the presence of 20 microM arachidonic acid. BCAR, with damaged endothelium, produced significantly less PGI2 than that with intact endothelium. However, co-incubation with CGS 13080-treated platelets resulted in an increase in PGI2 independent of endothelium, demonstrating a shunt of PGH2 from platelets to BCAR. Co-incubation of BCAR with aspirin-treated platelets resulted in a net increase in TXA2 demonstrating a shunt of PGH2 from BCAR to platelets. Employing [14C]PGH2 as substrate, BCAR with and without intact endothelium produced similar amounts of 6-keto-[14C]PGF1 alpha. Likewise, homogenates (50 micrograms protein) of intimal and subintimal regions of BCAR and BCAR converted similar amounts of PGH2 to 6-keto-PGF1 alpha. These data suggest that vascular production of PGH2 is more dependent on an intact endothelium than is the conversion of PGH2 to PGI2. These data also suggest a potential for a bidirectional exchange of PGH2 between platelets and vascular wall during platelet-vascular wall interactions.
Authors:
P R Mayeux; P J Kadowitz; D B McNamara
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1011     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1989 Mar 
Date Detail:
Created Date:  1989-05-05     Completed Date:  1989-05-05     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  18-24     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, Tulane University School of Medicine, New Orleans, LA 70112.
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MeSH Terms
Descriptor/Qualifier:
6-Ketoprostaglandin F1 alpha / biosynthesis
Animals
Arachidonic Acid
Arachidonic Acids / metabolism
Aspirin / pharmacology
Blood Platelets / drug effects,  metabolism*
Cattle
Coronary Vessels / metabolism*
Cyclooxygenase Inhibitors
Cytochrome P-450 Enzyme System / metabolism
Epoprostenol / biosynthesis
Humans
Imidazoles / pharmacology
Intramolecular Oxidoreductases*
Isomerases / metabolism
Prostaglandin Endoperoxides / metabolism*
Prostaglandin Endoperoxides, Synthetic / metabolism
Prostaglandin H2
Prostaglandins H / metabolism
Pyridines / pharmacology
Thromboxane B2 / biosynthesis
Thromboxane-A Synthase / antagonists & inhibitors
Grant Support
ID/Acronym/Agency:
HL15580/HL/NHLBI NIH HHS; HL18070/HL/NHLBI NIH HHS; HL29456/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Arachidonic Acids; 0/Cyclooxygenase Inhibitors; 0/Imidazoles; 0/Prostaglandin Endoperoxides; 0/Prostaglandin Endoperoxides, Synthetic; 0/Prostaglandins H; 0/Pyridines; 35121-78-9/Epoprostenol; 42935-17-1/Prostaglandin H2; 50-78-2/Aspirin; 506-32-1/Arachidonic Acid; 54397-85-2/Thromboxane B2; 58962-34-8/6-Ketoprostaglandin F1 alpha; 85691-74-3/pirmagrel; 9035-51-2/Cytochrome P-450 Enzyme System; EC 5.-/Isomerases; EC 5.3.-/Intramolecular Oxidoreductases; EC 5.3.99.4/prostacyclin synthetase; EC 5.3.99.5/Thromboxane-A Synthase

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