| Evidence for basic fibroblast growth factor receptors in cultured immature Leydig cells. | |
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MedLine Citation:
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PMID: 1459339 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Previous studies have shown that basic fibroblast growth factor (bFGF) can modulate basal and luteinizing hormone/human chorionic gonadotropin (LH/hCG)-stimulated Leydig cell functions. It has not been ascertained whether these actions are due to direct or indirect effects on Leydig cells. To resolve this question, a multi-step procedure was used to isolate highly-purified Leydig cells from immature rats. 125I-bFGF binding studies were performed on cultured cells. Scatchard analysis of the data indicated a single binding site with an apparent Kd of 82 pM and a binding capacity of approximately 2800 sites per cell. Both bFGF and acidic FGF similarly were effective in displacing 125I-bFGF, suggesting that the receptor binds both bFGF and aFGF. However, neither hCG, follicle-stimulating hormone (FSH), insulin, insulin-like growth factor-1 (IGF-1), prolactin, platelet-derived growth factor (PDGF) or epidermal growth factor (EGF) were effective competitors. When binding studies were conducted on cultured testicular interstitial cellular fractions that are normally discarded during Leydig cell purification, bFGF receptors were identified in these fractions. These results demonstrate that bFGF can have direct effects on Leydig cells through specific receptors; however, because other interstitial cell type(s) also have bFGF receptors, they stress the importance of using highly purified cells when evaluating bFGF actions on Leydig cells. |
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Authors:
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E P Murono; A L Washburn; D P Goforth; N Wu |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Molecular and cellular endocrinology Volume: 88 ISSN: 0303-7207 ISO Abbreviation: Mol. Cell. Endocrinol. Publication Date: 1992 Oct |
Date Detail:
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Created Date: 1993-01-08 Completed Date: 1993-01-08 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7500844 Medline TA: Mol Cell Endocrinol Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 39-45 Citation Subset: IM |
Affiliation:
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Research Service, Dorn Veterans' Hospital, Columbia, SC. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Binding, Competitive Cells, Cultured Fibroblast Growth Factor 2 / metabolism* Hormones / metabolism Kinetics Leydig Cells / metabolism* Male Rats Receptors, Fibroblast Growth Factor / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Hormones; 0/Receptors, Fibroblast Growth Factor; 103107-01-3/Fibroblast Growth Factor 2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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