Document Detail

Evidence of association of the ecNOS gene polymorphism with plasma NO metabolite levels in humans.
MedLine Citation:
PMID:  9535806     Owner:  NLM     Status:  MEDLINE    
Nitric oxide (NO) synthesized by the vascular endothelium regulates mammalian blood vessels and other systems in humans. Recently, an endothelial nitric oxide synthase (ecNOS) gene polymorphism, the 27-bp repeat in intron 4 (ecNOS4), was reported to be related to the pathogenesis of coronary heart disease and terminal renal failure. We analyzed this polymorphism in a group of 413 healthy subjects, and measured their plasma nitrite and nitrate (NOx) levels. The frequency of the b allele was 89.8% , and the frequency of the a allele was 10.2%. The frequency of ecNOS4 b/b, ecNOS4 b/a, and ecNOS4 a/a in the healthy subjects in this study was 0.814 (n=336), 0.169 (n=70) and 0.017 (n=7), respectively. Using this polymorphism as a DNA marker, we found a strong association between the alleles of the ecNOS gene polymorphism and the plasma NOx levels. The basal NO metabolite levels were 28.8 micromol/L in subjects with ecNOS4 a/a, 31.4 micromol/L in those with ecNOS4 b/a, and 35.5 micromol/L in those with ecNOS4 b/b. The mean plasma NOx level of the subjects who were homozygous for the a allele was nearly 20% lower than in the subjects with the b allele. The plasma NOx level of the subjects with the a allele was 31.2+/-2.00 micromol/L, and significantly lower than the 35.5+/-0.93 micromol/L in those without the a allele (P <0.05). The results of this study indicate that the ecNOS4 gene locus may be responsible for variations in the genetic control of plasma NOx and that analysis of ecNOS4 gene polymorphism may be a useful tool for studying the relation between NO and diseases.
T Tsukada; K Yokoyama; T Arai; F Takemoto; S Hara; A Yamada; Y Kawaguchi; T Hosoya; J Igari
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  245     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1998 Apr 
Date Detail:
Created Date:  1998-05-14     Completed Date:  1998-05-14     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  190-3     Citation Subset:  IM    
Copyright Information:
Copyright 1998 Academic Press.
Department of Clinical Chemistry, Toranomon Hospital, Tokyo, 105, Japan.
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MeSH Terms
Coronary Disease / physiopathology
Genetic Markers / genetics
Kidney Failure, Chronic / physiopathology
Middle Aged
Nitrates / blood
Nitric Oxide / blood*
Nitric Oxide Synthase / genetics*
Nitrites / blood
Polymorphism, Genetic / genetics*
Reg. No./Substance:
0/Genetic Markers; 0/Nitrates; 0/Nitrites; 10102-43-9/Nitric Oxide; EC Oxide Synthase

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