Document Detail

Evidence for a Na+/H+ antiport in stomach smooth muscle cells.
MedLine Citation:
PMID:  2831889     Owner:  NLM     Status:  MEDLINE    
Single smooth muscle cells were isolated from circular muscle of the canine gastric corpus by collagenase incubation. Cytoplasmic pH (pHi) of these cells was measured fluorometrically using the trapped dye 2',7'-bis(carboxyethyl)-5,6-carboxyfluorescein. Cells were examined for their Na+/H+ exchange activity after intracellular acidification. Cells acid-loaded by propionate exposure, the NH4+ prepulse technique or suspension in a Na+-depleted medium regained almost normal pHi upon exposure to a Na+ medium. The Na+-dependent alkalinization was amiloride sensitive. As well, addition of amiloride to cells suspended in a Na+ medium caused a concurrent decrease in pHi. The study indicates that a Na+/H+ antiport is present in these smooth muscle cells.
T J Finkle; A K Grover
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  151     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1988 Feb 
Date Detail:
Created Date:  1988-04-11     Completed Date:  1988-04-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  473-9     Citation Subset:  IM    
Department of Neurosciences, McMaster University Health Sciences Centre, Hamilton, Ontario, Canada.
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MeSH Terms
Amiloride / pharmacology
Ammonium Chloride / metabolism
Carrier Proteins / analysis*
Cells, Cultured
Culture Media
Cytoplasm / metabolism
Hydrogen-Ion Concentration
Muscle, Smooth / analysis*,  cytology,  metabolism,  ultrastructure
Propionates / metabolism
Sodium / metabolism
Sodium Chloride / pharmacology
Sodium-Hydrogen Antiporter
Stomach / analysis*,  cytology,  metabolism
Reg. No./Substance:
0/Carrier Proteins; 0/Culture Media; 0/Propionates; 0/Sodium-Hydrogen Antiporter; 12125-02-9/Ammonium Chloride; 2609-46-3/Amiloride; 7440-23-5/Sodium; 7647-14-5/Sodium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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