| Evidence against a functional site for Bcl-2 downstream of caspase cascade in preventing apoptosis. | |
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MedLine Citation:
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PMID: 9365238 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Apoptotic cell death is driven by ICE family proteases (caspases) and negatively regulated by Bcl-2 family proteins. Although it has been shown that Bcl-2 exerts anti-apoptotic activity by blocking a step(s) leading to the activation of caspases, a role for Bcl-2 and Bcl-xL downstream of the caspase cascade has remained unclear. Here, we show that purified active caspase-3 (CPP32/Yama/apopain) and caspase-1 (ICE) induces apoptosis when microinjected into the cytoplasm of cells, confirming our recent observations, and that the apoptosis is not at all prevented by Bcl-2 and Bcl-xL, which are overexpressed more than sufficiently to prevent Fas-mediated and overexpressed procaspase-1-mediated apoptosis. Thus, Bcl-2 and Bcl-xL do not act downstream of the caspase cascade. |
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Authors:
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N Yasuhara; S Sahara; S Kamada; Y Eguchi; Y Tsujimoto |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Oncogene Volume: 15 ISSN: 0950-9232 ISO Abbreviation: Oncogene Publication Date: 1997 Oct |
Date Detail:
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Created Date: 1997-12-04 Completed Date: 1997-12-04 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8711562 Medline TA: Oncogene Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 1921-8 Citation Subset: IM |
Affiliation:
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Department of Medical Genetics, Biomedical Research Center, Osaka University Medical School, Suita, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis* Cysteine Endopeptidases / metabolism* Cytoplasm / metabolism Enzyme Precursors / metabolism Hela Cells Humans Microinjections Proto-Oncogene Proteins c-bcl-2 / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Precursors; 0/Proto-Oncogene Proteins c-bcl-2; EC 3.4.22.-/Cysteine Endopeptidases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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