Document Detail


Evidence against electrophoresis as the principal mode of protein transport in vitellogenic ovarian follicles of Drosophila.
MedLine Citation:
PMID:  3446477     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Charged cell constituents in polytrophic insect follicles are thought to be transported in the nurse cell-oocyte syncytium by way of electrophoresis. This concept, proposed by Woodruff & Telfer (1980) was based on electrophysiological data and microinjection of heterologous proteins using Hyalophora follicles. By microinjecting fluorescently labelled acidic and basic proteins into the nurse cells or oocyte of vitellogenic Drosophila follicles, we failed to obtain evidence for charge-dependent migration of these molecules. We have also analyzed the proteins of nurse cells and oocyte on isoelectric focusing gels, by means of two-dimensional gel electrophoresis, and by ion exchange chromatography to see if basic or acidic proteins accumulate in vivo in nurse cells and oocyte, respectively. For the bulk of the follicular proteins we found no accumulation. Further evidence against an electrophoretic transport system in Drosophila was obtained by estimating the intracellular pH from the colour of indicator dyes microinjected into the follicles; the results indicate that the pH in the nurse cell cytoplasm is lower than that in the ooplasm. According to the model developed for Hyalophora, electrophoretic transport would be favoured by high pH in the nurse cell cytoplasm.
Authors:
J Bohrmann; H Gutzeit
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  101     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  1987 Oct 
Date Detail:
Created Date:  1988-05-24     Completed Date:  1988-05-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  279-88     Citation Subset:  IM    
Affiliation:
Biologisches Institut I (Zoologie), Universität Freiburg, Federal Republic of Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Transport
Chromatography, Ion Exchange
Drosophila / metabolism*
Female
Hydrogen-Ion Concentration
Isoelectric Focusing
Microinjections
Microscopy, Fluorescence
Oocytes / metabolism
Ovary / metabolism*
Proteins / pharmacokinetics*
Chemical
Reg. No./Substance:
0/Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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