Document Detail


Evidence for two promoters internal to the alginate biosynthesis operon in Pseudomonas aeruginosa.
MedLine Citation:
PMID:  22986819     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
While much is known about the transcriptional regulation of the 12 gene alginate biosynthesis operon from the algD promoter in Pseudomonas aeruginosa, there has been little investigation into the possibility of other transcription starts within this operon, especially those genes dealing with the epimerization and acetylation of the alginate polymer. In this study, we utilized quantitative reverse transcription polymerase chain reaction, a β-galactosidase reporter assay and sequence scanning to identify two putative promoters within the alginate biosynthesis operon upstream of the alginate epimerase gene algG and the alginate acetylation gene algI. These data support the possibility of differential regulation within the operon to alter polymer structure under varying environmental conditions.
Authors:
Janice L Paletta; Dennis E Ohman
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-09-18
Journal Detail:
Title:  Current microbiology     Volume:  65     ISSN:  1432-0991     ISO Abbreviation:  Curr. Microbiol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-10-22     Completed Date:  2013-04-29     Revised Date:  2014-10-04    
Medline Journal Info:
Nlm Unique ID:  7808448     Medline TA:  Curr Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  770-5     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acetylation
Alginates / metabolism*
Bacterial Proteins / genetics,  metabolism*
Base Sequence
Culture Media
Gene Expression Regulation, Bacterial*
Genes, Reporter
Molecular Sequence Data
Operon*
Promoter Regions, Genetic / genetics*
Pseudomonas aeruginosa / genetics,  growth & development,  metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Sequence Alignment
Sequence Analysis, DNA
beta-Galactosidase / genetics
Grant Support
ID/Acronym/Agency:
AI-19146/AI/NIAID NIH HHS; R01 AI019146/AI/NIAID NIH HHS; T32 AI007617/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Alginates; 0/Bacterial Proteins; 0/Culture Media; EC 3.2.1.23/beta-Galactosidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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