| Evidence for rosettes as an unrecognized stage in the life cycle of Leishmania parasites. | |
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MedLine Citation:
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PMID: 20707828 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Leishmania parasites, which afflict 12 million people in 88 countries, exist as promastigotes transmitted by insect vectors and as amastigotes residing in mammalian macrophages. Promastigote cells arranged in rosettes have also been described but universally disregarded as a distinct stage in the life cycle. We present evidence that only rosettes of Leishmania major promastigotes express cell surface poly-alpha2,8 N-acetyl neuraminic acid (PSA) and PSA containing de-N-acetyl neuraminic acid (NeuPSA). Expression of rosette-specific PSA antigens was mosaic, with individual promastigotes expressing PSA, NeuPSA or both. A 50 kDa protein was detected by Western blot analysis of a detergent-insoluble cell fraction with both PSA and NeuPSA-reactive antibodies. Frequencies of rosette formation as well as cell surface PSA/NeuPSA expression were temperature dependent. Rosettes also engaged in an unusual swarming behavior, congregating into extended clusters. Distinct structures resembling cellular fusion bodies were formed in and released from rosettes. The results indicate that rosettes are an unrecognized stage in the life cycle of Leishmania. We hypothesize that rosettes initiate mating in Leishmania during which PSA/NeuPSA expression plays an important role. Recognizing rosettes as a distinct form of the Leishmania life cycle opens new possibilities for treatment or prevention of disease and, possibly, in vitro genetic recombination without passage of cells through insect vectors. |
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Authors:
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David M Iovannisci; C Paul Plested; Gregory R Moe |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-08-05 |
Journal Detail:
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Title: The Journal of eukaryotic microbiology Volume: 57 ISSN: 1550-7408 ISO Abbreviation: J. Eukaryot. Microbiol. Publication Date: 2010 Sep-Oct |
Date Detail:
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Created Date: 2010-08-26 Completed Date: 2010-12-21 Revised Date: 2011-09-13 |
Medline Journal Info:
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Nlm Unique ID: 9306405 Medline TA: J Eukaryot Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 405-14 Citation Subset: IM |
Affiliation:
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Center for Immunobiology and Vaccine Development, Children's Hospital and Research Center Oakland, Oakland, California 94609, USA. diovannisci@chori.org |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, Protozoan
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analysis,
genetics,
metabolism Cell Adhesion Leishmania major / chemistry*, genetics, growth & development*, physiology Microscopy, Confocal |
| Grant Support | |
ID/Acronym/Agency:
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CO6 RR-16226/CO/NCI NIH HHS; R01 AI064314-04/AI/NIAID NIH HHS; R01 AI46464/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Protozoan |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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