Document Detail


Evidence of Ongoing Replication in a Human Immunodeficiency Virus Type 1 Persistently Infected Cell Line.
MedLine Citation:
PMID:  23288426     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
ABSTRACT Human immunodeficiency virus type 1 (HIV-1) persistently infected cell lines are characterized by the continuous viral production without cytopathic effect. However, it is not completely clear if this production is contributed only by viral transcription or also by new cycles of viral replication. We studied an HIV-1 persistently infected cell line, designated H61-D, providing evidence of new replication cycles as sustained by: i) a decrease in viral production, measured by p24 protein, after treatment of the culture with 3'-Azydo-3'-Deoxythymydine (AZT); ii) detection of new integration events in the course of cell culture, and iii) finding of two-long terminal repeat (2-LTR) circles in the cells. H61-D cells were not infected by cell free virus, but infection was possible by co-culture with another productive infected cell line. In conclusion, ongoing viral replication is taking place in H61-D persistent cells and new infections are mediated by a cell-to-cell spread mechanism.
Authors:
Isabel Olivares; Carmen Sanchez-Jimenez; Catarina Reis Vieira; Victor Toledano; Mónica Gutierrez-Rivas; Cecilio López-Galíndez
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-3
Journal Detail:
Title:  The Journal of general virology     Volume:  -     ISSN:  1465-2099     ISO Abbreviation:  J. Gen. Virol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0077340     Medline TA:  J Gen Virol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
INSTITUTO DE SALUD CARLOS III, MADRID, SPAIN.
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