Document Detail


Evidence for MoeA-dependent formation of the molybdenum cofactor from molybdate and molybdopterin in Escherichia coli.
MedLine Citation:
PMID:  12420167     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The function of the MoeA protein in the biosynthesis of the molybdenum cofactor (MoCo) was analyzed in vitro, using purified His(6)-MoeA from Escherichia coli, molybdopterin (MPT) isolated from buttermilk xanthine oxidase and molybdate. The formation of MoCo was monitored by the reconstitution of nitrate reductase activity in extracts of the Neurospora crassa nit-1 mutant. Formation of MoCo from MPT and molybdate required MoeA and L-cysteine or glutathione. The reaction proceeded at micromolar molybdate levels and was time- and MoeA concentration-dependent. A physical interaction between MoeA and MPT was demonstrated by HPLC analysis of MoeA-bound MPT.
Authors:
Cristinel Sandu; Roderich Brandsch
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2002-09-03
Journal Detail:
Title:  Archives of microbiology     Volume:  178     ISSN:  0302-8933     ISO Abbreviation:  Arch. Microbiol.     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2002-11-06     Completed Date:  2003-06-10     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0410427     Medline TA:  Arch Microbiol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  465-70     Citation Subset:  IM    
Affiliation:
Institut für Biochemie und Molekularbiologie, Universität Freiburg i. Br., Hermann-Herder-Strasse 7, 79104 Freiburg, Germany.
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MeSH Terms
Descriptor/Qualifier:
Chromatography, High Pressure Liquid
Coenzymes / biosynthesis
Escherichia coli / metabolism*
Escherichia coli Proteins*
Metalloproteins / metabolism*
Molybdenum / metabolism*
Neurospora crassa / genetics,  metabolism
Nitrate Reductases / metabolism
Pteridines / metabolism*
Sulfurtransferases / metabolism*
Xanthine Oxidase / metabolism
Chemical
Reg. No./Substance:
0/Coenzymes; 0/Escherichia coli Proteins; 0/Metalloproteins; 0/Pteridines; 14259-85-9/molybdate; 73508-07-3/molybdenum cofactor; 7439-98-7/Molybdenum; EC 1.17.3.2/Xanthine Oxidase; EC 1.7.-/Nitrate Reductases; EC 2.8.1.-/MoeA protein, E coli; EC 2.8.1.-/Sulfurtransferases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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