Document Detail

Evidence for MoeA-dependent formation of the molybdenum cofactor from molybdate and molybdopterin in Escherichia coli.
MedLine Citation:
PMID:  12420167     Owner:  NLM     Status:  MEDLINE    
The function of the MoeA protein in the biosynthesis of the molybdenum cofactor (MoCo) was analyzed in vitro, using purified His(6)-MoeA from Escherichia coli, molybdopterin (MPT) isolated from buttermilk xanthine oxidase and molybdate. The formation of MoCo was monitored by the reconstitution of nitrate reductase activity in extracts of the Neurospora crassa nit-1 mutant. Formation of MoCo from MPT and molybdate required MoeA and L-cysteine or glutathione. The reaction proceeded at micromolar molybdate levels and was time- and MoeA concentration-dependent. A physical interaction between MoeA and MPT was demonstrated by HPLC analysis of MoeA-bound MPT.
Cristinel Sandu; Roderich Brandsch
Related Documents :
2417437 - Location of the angiogenic activity in the pregnant human uterus.
9038997 - Effect of hypoxemia on tissue glycogen content and glycolytic enzyme activities in feta...
13953737 - Increased sensitivity of taste and smell in cystic fibrosis.
20504907 - Pregnancy and the endocrine regulation of the baroreceptor reflex.
1803307 - Alternate methods of first-trimester diagnosis.
19217907 - Toxoplasma gondii: congenital transmission in a hamster model.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2002-09-03
Journal Detail:
Title:  Archives of microbiology     Volume:  178     ISSN:  0302-8933     ISO Abbreviation:  Arch. Microbiol.     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2002-11-06     Completed Date:  2003-06-10     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0410427     Medline TA:  Arch Microbiol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  465-70     Citation Subset:  IM    
Institut für Biochemie und Molekularbiologie, Universität Freiburg i. Br., Hermann-Herder-Strasse 7, 79104 Freiburg, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Chromatography, High Pressure Liquid
Coenzymes / biosynthesis
Escherichia coli / metabolism*
Escherichia coli Proteins*
Metalloproteins / metabolism*
Molybdenum / metabolism*
Neurospora crassa / genetics,  metabolism
Nitrate Reductases / metabolism
Pteridines / metabolism*
Sulfurtransferases / metabolism*
Xanthine Oxidase / metabolism
Reg. No./Substance:
0/Coenzymes; 0/Escherichia coli Proteins; 0/Metalloproteins; 0/Pteridines; 14259-85-9/molybdate; 73508-07-3/molybdenum cofactor; 7439-98-7/Molybdenum; EC Oxidase; EC 1.7.-/Nitrate Reductases; EC 2.8.1.-/MoeA protein, E coli; EC 2.8.1.-/Sulfurtransferases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Influence of nitrate on oxalate- and glyoxylate-dependent growth and acetogenesis by Moorella thermo...
Next Document:  The roles of the three gene copies encoding hydroxylamine oxidoreductase in Nitrosomonas europaea.