| Evidence of increased oxidative stress in aged mesenteric lymphatic vessels. | |
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MedLine Citation:
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PMID: 22540739 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: We have previously shown that aging is associated with weakened rat mesenteric lymphatic vessel (MLV) contractility. However, the specific mechanisms contributing to this aging-associated contractile degeneration remain unknown. Aging is often associated with elevations in oxidative stress, and reactive oxygen species (ROS) have been shown to reduce the contractility of MLV. Thus in the present study, we sought to assess whether aging is associated with increased levels of oxidative stress and oxidative damage in MLV. METHODS AND RESULTS: MLV were isolated from 9-mo- and 24-mo-old Fischer-344 rats and subjected to the following experimental techniques: measurement of total superoxide dismutase (SOD) activity; estimation of lipid peroxidation levels via measurement of thiobarbituric acid reactive substances (TBARS); detection of superoxide and mitochondrial ROS in live MLV; Western blot analysis, and immunohistochemical labeling of the SOD isoforms and nitro-tyrosine proteins. We found that aging is associated with increased levels of cellular superoxide and mitochondrial ROS concomitant with a reduction in Cu/Zn-SOD protein expression and total SOD enzymatic activity in MLV. This increase in oxidative stress and decrease in antioxidant activity was associated with evidence of increased lipid (as indicated by TBARS) and protein (as indicated by nitro-tyrosine labeling) oxidative damage. CONCLUSIONS: Thus for the first time, we demonstrate that aging-associated increases in oxidative stress and oxidative damage is indeed present in the walls of MLV and may contribute to the aging-associated lymphatic pump dysfunction we previously reported. |
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Authors:
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Sangeetha Thangaswamy; Eric A Bridenbaugh; Anatoliy A Gashev |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2012-04-27 |
Journal Detail:
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Title: Lymphatic research and biology Volume: 10 ISSN: 1557-8585 ISO Abbreviation: Lymphat Res Biol Publication Date: 2012 Jun |
Date Detail:
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Created Date: 2012-06-22 Completed Date: 2012-10-19 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 101163587 Medline TA: Lymphat Res Biol Country: United States |
Other Details:
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Languages: eng Pagination: 53-62 Citation Subset: IM |
Affiliation:
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Department of Systems Biology and Translational Medicine, College of Medicine, Texas A&M Health Science Center, Temple, Texas 76504, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Age Factors Aging / metabolism* Animals Blotting, Western Immunohistochemistry Lipid Peroxidation Lymphatic Vessels / enzymology, metabolism* Male Mesentery / enzymology, metabolism* Mitochondria / metabolism Oxidative Stress* Rats Rats, Inbred F344 Reactive Oxygen Species / metabolism Superoxide Dismutase / metabolism Superoxides / metabolism Thiobarbituric Acid Reactive Substances / metabolism Tyrosine / analogs & derivatives, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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HL-094269/HL/NHLBI NIH HHS; NIH RO1 AG-030578/AG/NIA NIH HHS; R01 AG030578/AG/NIA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Reactive Oxygen Species; 0/Thiobarbituric Acid Reactive Substances; 11062-77-4/Superoxides; 3604-79-3/3-nitrotyrosine; 55520-40-6/Tyrosine; EC 1.15.1.-/superoxide dismutase 1; EC 1.15.1.1/Sod3 protein, rat; EC 1.15.1.1/Superoxide Dismutase; EC 1.15.1.1/superoxide dismutase 2 |
| Comments/Corrections | |
Comment In:
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Lymphat Res Biol. 2012 Jun;10(2):45
[PMID:
22720660
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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