Document Detail

Evidence of increased oxidative stress in aged mesenteric lymphatic vessels.
MedLine Citation:
PMID:  22540739     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: We have previously shown that aging is associated with weakened rat mesenteric lymphatic vessel (MLV) contractility. However, the specific mechanisms contributing to this aging-associated contractile degeneration remain unknown. Aging is often associated with elevations in oxidative stress, and reactive oxygen species (ROS) have been shown to reduce the contractility of MLV. Thus in the present study, we sought to assess whether aging is associated with increased levels of oxidative stress and oxidative damage in MLV.
METHODS AND RESULTS: MLV were isolated from 9-mo- and 24-mo-old Fischer-344 rats and subjected to the following experimental techniques: measurement of total superoxide dismutase (SOD) activity; estimation of lipid peroxidation levels via measurement of thiobarbituric acid reactive substances (TBARS); detection of superoxide and mitochondrial ROS in live MLV; Western blot analysis, and immunohistochemical labeling of the SOD isoforms and nitro-tyrosine proteins. We found that aging is associated with increased levels of cellular superoxide and mitochondrial ROS concomitant with a reduction in Cu/Zn-SOD protein expression and total SOD enzymatic activity in MLV. This increase in oxidative stress and decrease in antioxidant activity was associated with evidence of increased lipid (as indicated by TBARS) and protein (as indicated by nitro-tyrosine labeling) oxidative damage.
CONCLUSIONS: Thus for the first time, we demonstrate that aging-associated increases in oxidative stress and oxidative damage is indeed present in the walls of MLV and may contribute to the aging-associated lymphatic pump dysfunction we previously reported.
Sangeetha Thangaswamy; Eric A Bridenbaugh; Anatoliy A Gashev
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-04-27
Journal Detail:
Title:  Lymphatic research and biology     Volume:  10     ISSN:  1557-8585     ISO Abbreviation:  Lymphat Res Biol     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-06-22     Completed Date:  2012-10-19     Revised Date:  2013-06-25    
Medline Journal Info:
Nlm Unique ID:  101163587     Medline TA:  Lymphat Res Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  53-62     Citation Subset:  IM    
Department of Systems Biology and Translational Medicine, College of Medicine, Texas A&M Health Science Center, Temple, Texas 76504, USA.
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MeSH Terms
Age Factors
Aging / metabolism*
Blotting, Western
Lipid Peroxidation
Lymphatic Vessels / enzymology,  metabolism*
Mesentery / enzymology,  metabolism*
Mitochondria / metabolism
Oxidative Stress*
Rats, Inbred F344
Reactive Oxygen Species / metabolism
Superoxide Dismutase / metabolism
Superoxides / metabolism
Thiobarbituric Acid Reactive Substances / metabolism
Tyrosine / analogs & derivatives,  metabolism
Grant Support
Reg. No./Substance:
0/Reactive Oxygen Species; 0/Thiobarbituric Acid Reactive Substances; 11062-77-4/Superoxides; 3604-79-3/3-nitrotyrosine; 55520-40-6/Tyrosine; EC 1.15.1.-/superoxide dismutase 1; EC protein, rat; EC Dismutase; EC dismutase 2
Comment In:
Lymphat Res Biol. 2012 Jun;10(2):45   [PMID:  22720660 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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