| Evidence for endotoxin contamination in plastic Na+-heparin blood collection tube lots. | |
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MedLine Citation:
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PMID: 20663962 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Biomarker assays are often conducted on whole blood samples in the course of drug development studies. Because bacterial lipopolysaccharide (LPS) (endotoxin) contamination is known to cause spontaneous cytokine production by monocytes, contamination of blood collection tubes may interfere with biomarker assay results. METHODS: Whole blood from healthy donors was collected into plastic or glass sodium (Na(+))-heparin Vacutainer() blood collection tubes and heparinized syringes. Samples were analyzed for phosphoprotein response, cytokine production, and RNA expression. Tubes were tested for endotoxin contamination by use of the limulus amoebocyte lysate assay. RESULTS: Results of phospho-flow cytometry, branched DNA (bDNA), and ELISA assays indicated that a specific lot (#5339582) of plastic Na(+)-heparin Vacutainer tubes was highly contaminated with an endotoxinlike substance, and contamination was confirmed by the limulus amoebocyte lysate assay. Analysis of multiple-analyte panels revealed that analytes whose changed expression was predictive of LPS stimulation were increased when whole blood was incubated in contaminated tubes for 6 or 18 h. Two additional lots of plastic tubes tested had detectable amounts of endotoxin sufficient to strongly alter phospho-flow cytometry analyses, as determined by the fold change in phosphorylation of p38 mitogen-activated protein kinase in response to tumor necrosis factor alpha and LPS. In contrast, 3 lots of glass tubes had substantially lower levels of spontaneous blood activation. CONCLUSIONS: Endotoxin contamination associated with tubes from 3 lots of a particular type of plastic Na(+)-heparin Vacutainer tube dramatically affected biomarker assay measurements. Prescreening these tubes is suggested before their use in clinical sample analysis. |
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Authors:
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Kathryn J Newhall; Geoffrey S Diemer; Natalia Leshinsky; Keith Kerkof; Hilary T Chute; Chris B Russell; William Rees; Andrew A Welcher; Scott D Patterson; Gary D Means |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-07-27 |
Journal Detail:
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Title: Clinical chemistry Volume: 56 ISSN: 1530-8561 ISO Abbreviation: Clin. Chem. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-08-30 Completed Date: 2010-09-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9421549 Medline TA: Clin Chem Country: United States |
Other Details:
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Languages: eng Pagination: 1483-91 Citation Subset: IM |
Affiliation:
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Amgen Molecular Sciences, Amgen, Seattle, WA, USA. knewhall@amgen.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Anticoagulants* Biological Markers / blood Blood Specimen Collection / instrumentation* C-Reactive Protein / biosynthesis, genetics Chemokine CCL2 / blood, genetics Chemokine CCL7 / blood, genetics Endotoxins / analysis* Enzyme-Linked Immunosorbent Assay Equipment Contamination* Flow Cytometry Heparin* Humans Interleukin-1beta / blood Interleukin-6 / blood, genetics Lipopolysaccharides / pharmacology Phosphorylation Plastics RNA, Messenger / blood Serum Amyloid P-Component / biosynthesis, genetics Time Factors Tumor Necrosis Factor-alpha / biosynthesis, pharmacology p38 Mitogen-Activated Protein Kinases / blood |
| Chemical | |
Reg. No./Substance:
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0/Anticoagulants; 0/Biological Markers; 0/Chemokine CCL2; 0/Chemokine CCL7; 0/Endotoxins; 0/Interleukin-1beta; 0/Interleukin-6; 0/Lipopolysaccharides; 0/Plastics; 0/RNA, Messenger; 0/Serum Amyloid P-Component; 0/Tumor Necrosis Factor-alpha; 148591-49-5/PTX3 protein; 9005-49-6/Heparin; 9007-41-4/C-Reactive Protein; EC 2.7.11.24/p38 Mitogen-Activated Protein Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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