Document Detail

Evidence for a DIOA-sensitive [K+,Cl-]-cotransport system in cultured vascular smooth muscle cells.
MedLine Citation:
PMID:  2081015     Owner:  NLM     Status:  MEDLINE    
The existence of a [K+,Cl-]-cotransport system in vascular smooth muscle cells was investigated in the A10 cell line by studying the effect of DIOA (dihydroindenyl-oxy-alkanoic acid, a potent inhibitor) on K+, Rb+ and Cl- fluxes. Hypotonic medium (150 mOsm) increased initial rates of ouabain and bumetanide-resistant (OBR) Rb+ uptake by 100%, bumetanide and DIDS-resistant Cl- uptake by 200%, and OBR net K+ efflux by 130%. DIOA inhibited 40 to 100% of the Rb+ influx and net K+ efflux stimulation with an IC50 of 4 X 10(-5) mol/L. DIOA-sensitive Rb+ influx was a sigmoidal function of the decrease in osmolarity, with a threshold at about 230 mOsm. Our results suggest that vascular smooth muscle cells have a DIOA-sensitive [K+,Cl-]-cotransport system. Dissipation of the outwardly directed Cl- gradient with an apparent [Cl- to K+] stoichiometry much higher than one may provide the energy to ensure net KCl (and osmotic water) extrusion and cell volume regulation in these cells.
M Saitta; S Cavalier; R Garay; E Cragoe; P Hannaert
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  American journal of hypertension     Volume:  3     ISSN:  0895-7061     ISO Abbreviation:  Am. J. Hypertens.     Publication Date:  1990 Dec 
Date Detail:
Created Date:  1991-05-07     Completed Date:  1991-05-07     Revised Date:  2009-02-24    
Medline Journal Info:
Nlm Unique ID:  8803676     Medline TA:  Am J Hypertens     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  939-42     Citation Subset:  IM    
Institut National de la Santé et de la Recherche Médicale U7/Centre National de la Recherche Scientifique UA 318. Hôpital Necker, Paris, France.
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MeSH Terms
Biological Transport, Active / drug effects
Carboxylic Acids / pharmacology
Carrier Proteins / metabolism
Cell Line
Chlorides / metabolism*
Indenes / pharmacology
Muscle, Smooth, Vascular / drug effects,  metabolism*
Osmolar Concentration
Potassium / metabolism*
Rubidium / metabolism
Reg. No./Substance:
0/((dihydroindenyl)oxy)alkanoic acid; 0/Carboxylic Acids; 0/Carrier Proteins; 0/Chlorides; 0/Indenes; 0/Symporters; 0/potassium-chloride symporters; 7440-09-7/Potassium; 7440-17-7/Rubidium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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