Document Detail


Evidence that CXCL16 is a potent mediator of angiogenesis and is involved in endothelial progenitor cell chemotaxis : studies in mice with K/BxN serum-induced arthritis.
MedLine Citation:
PMID:  23633118     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To examine the possibility that CXCL16 recruits endothelial cells (ECs) to developing neovasculature in rheumatoid arthritis (RA) synovium.
METHODS: We utilized the RA synovial tissue SCID mouse chimera system to examine human microvascular EC (HMVEC) and human endothelial progenitor cell (EPC) recruitment into engrafted human synovium that was injected intragraft with CXCL16-immunodepleted RA synovial fluid (SF). CXCR6-deficient and wild-type (WT) C57BL/6 mice were primed to develop K/BxN serum-induced arthritis and evaluated for angiogenesis. HMVECs and EPCs from human cord blood were also examined for CXCR6 expression, by immunofluorescence and assessment of CXCL16 signaling activity.
RESULTS: CXCR6 was prominently expressed on human EPCs and HMVECs, and its expression on HMVECs could be up-regulated by interleukin-1β. SCID mice injected with CXCL16-depleted RA SF exhibited a significant reduction in EPC recruitment. In experiments using the K/BxN serum-induced inflammatory arthritis model, CXCR6(-/-) mice showed profound reductions in hemoglobin levels, which correlated with reductions in monocyte and T cell recruitment to arthritic joint tissue compared to that observed in WT mice. Additionally, HMVECs and EPCs responded to CXCL16 stimulation, but exhibited unique signal transduction pathways and homing properties.
CONCLUSION: These results indicate that CXCL16 and its receptor CXCR6 may be a central ligand/receptor pair that is closely associated with EPC recruitment and blood vessel formation in the RA joint.
Authors:
Takeo Isozaki; Ali S Arbab; Christian S Haas; M Asif Amin; Monica D Arendt; Alisa E Koch; Jeffrey H Ruth
Related Documents :
19292358 - The effects of suramin in prevention of peritendinous adhesions following flexor tendon...
1328308 - Ultrasonic detection of viscera slide as an indicator of abdominal wall adhesions.
10875868 - Post-operative consequences of glove powder used pre-operatively in the vagina in the r...
15589868 - Effect of adding more than 3% oxygen to carbon dioxide pneumoperitoneum on adhesion for...
15143208 - The airway compartment: chambers of secrets.
12067818 - Endothelial dysfunction in chronic heart failure: clinical and therapeutic implications.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Arthritis and rheumatism     Volume:  65     ISSN:  1529-0131     ISO Abbreviation:  Arthritis Rheum.     Publication Date:  2013 Jul 
Date Detail:
Created Date:  2013-07-03     Completed Date:  2013-09-04     Revised Date:  2014-07-01    
Medline Journal Info:
Nlm Unique ID:  0370605     Medline TA:  Arthritis Rheum     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1736-46     Citation Subset:  AIM; IM    
Copyright Information:
Copyright © 2013 by the American College of Rheumatology.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Arthritis, Experimental / metabolism*,  physiopathology
Arthritis, Rheumatoid / metabolism*,  physiopathology
Chemokine CXCL6 / physiology*
Chemokines, CXC / physiology*
Chemotaxis / physiology
Endothelial Cells / metabolism,  physiology*
Humans
Interleukin-1beta / pharmacology
Mice
Mice, Inbred C57BL
Mice, SCID
Mice, Transgenic
Neovascularization, Pathologic / metabolism*,  physiopathology
Receptors, CXCR / drug effects,  genetics,  physiology*
Receptors, Chemokine / metabolism,  physiology*
Receptors, Scavenger / physiology*
Receptors, Virus / metabolism,  physiology*
Signal Transduction / physiology
Stem Cells / physiology
Synovial Membrane / metabolism
Grant Support
ID/Acronym/Agency:
AI-40987/AI/NIAID NIH HHS; AR-48267/AR/NIAMS NIH HHS; HL-58694/HL/NHLBI NIH HHS; P30 AR048310/AR/NIAMS NIH HHS; P30-AR-048310/AR/NIAMS NIH HHS; R01 AI040987/AI/NIAID NIH HHS; R01 AR048267/AR/NIAMS NIH HHS; R01 CA122031/CA/NCI NIH HHS; R01 CA160216/CA/NCI NIH HHS; R01 CA172048/CA/NCI NIH HHS; R01 HL058695/HL/NHLBI NIH HHS; R01-CA-122031/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/CXCL16 protein, human; 0/CXCR6 protein, human; 0/Chemokine CXCL6; 0/Chemokines, CXC; 0/Cxcl16 protein, mouse; 0/Cxcr6 protein, mouse; 0/Interleukin-1beta; 0/Receptors, CXCR; 0/Receptors, Chemokine; 0/Receptors, Scavenger; 0/Receptors, Virus
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Latent Classes of Personality Disorders: Group Comparisons and Course of Psychotherapy.
Next Document:  Multidrug resistance-associated protein 2 (MRP2/ABCC2) haplotypes significantly affect the pharmacok...