Document Detail


An event-specific DNA microarray to identify genetically modified organisms in processed foods.
MedLine Citation:
PMID:  20438128     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We developed an event-specific DNA microarray system to identify 19 genetically modified organisms (GMOs), including two GM soybeans (GTS-40-3-2 and A2704-12), thirteen GM maizes (Bt176, Bt11, MON810, MON863, NK603, GA21, T25, TC1507, Bt10, DAS59122-7, TC6275, MIR604, and LY038), three GM canolas (GT73, MS8xRF3, and T45), and one GM cotton (LLcotton25). The microarray included 27 oligonucleotide probes optimized to identify endogenous reference targets, event-specific targets, screening targets (35S promoter and nos terminator), and an internal target (18S rRNA gene). Thirty-seven maize-containing food products purchased from South Korean and US markets were tested for the presence of GM maize using this microarray system. Thirteen GM maize events were simultaneously detected using multiplex PCR coupled with microarray on a single chip, at a limit of detection of approximately 0.5%. Using the system described here, we detected GM maize in 11 of the 37 food samples tested. These results suggest that an event-specific DNA microarray system can reliably detect GMOs in processed foods.
Authors:
Jae-Hwan Kim; Su-Youn Kim; Hyungjae Lee; Young-Rok Kim; Hae-Yeong Kim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  58     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-20     Completed Date:  2010-10-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6018-26     Citation Subset:  IM    
Affiliation:
Institute of Life Sciences & Resources and Graduate School of Biotechnology, Kyung Hee University, Yongin 446-701, Republic of Korea.
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MeSH Terms
Descriptor/Qualifier:
Brassica rapa / genetics
DNA, Plant / analysis
Food Analysis / methods*
Gossypium / genetics
Oligonucleotide Array Sequence Analysis / methods*
Oligonucleotide Probes
Plants, Genetically Modified / genetics*
Polymerase Chain Reaction
Soybeans / genetics
Zea mays / genetics
Chemical
Reg. No./Substance:
0/DNA, Plant; 0/Oligonucleotide Probes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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