Document Detail

Evaluation of use of a dicationic liquid stationary phase in the fast and conventional gas chromatographic analysis of health-hazardous C18 cis/trans fatty acids.
MedLine Citation:
PMID:  19480396     Owner:  NLM     Status:  MEDLINE    
The present research is focused on the evaluation of one 0.10 mm i.d. and two 0.25 mm i.d., ionic liquid (IL) stationary phase [1,9-di(3-vinyl-imidazolium) nonane bis(trifluoromethyl) sulfonyl imidate] columns, with lengths of 12 (the microbore capillary), 30 and 100 m, in the GC analysis of cis/trans fatty acid methyl esters (FAMEs). The selectivity of the IL columns toward a series of standard C(18:1), C(18:2), and C(18:3) geometric isomers (a group of 22 compounds was subjected to GC analysis) was compared to the performance of a widely used column in the cis/trans FAMEs analysis field, viz., a 100 m x 0.25 mm i.d. capillary with a 0.20 microm stationary phase film of bis-cyanopropyl polysiloxane (SP-2560). The selectivity provided by the IL phase was superior if compared to that of the other well-established capillary. An optimized IL method, using the longer column, was subjected to validation: retention time and peak area intraday precision (n = 5) were good, with RSD values lower than 0.07% and 6.6%, respectively; LODs (considering a S/N of 3) for C(18:1Delta)(9tr) and C(18:2Delta)(9tr,12tr) were 0.15 (7.3 ppm) and 0.18 ng (9.1 ppm) on-column, respectively, while LOQs (considering a S/N of 10) were 0.49 (24.3 ppm) and 0.60 ng (30.2 ppm), respectively; the method was found to be linear, for both trans FAMEs, in the 10-2000 ppm range. For the evaluation of accuracy, a hydrogenated margarine, spiked with known amounts of C(18:3Delta)(9c,12c,15c), was subjected to analysis using C(13:0) as an internal standard.
Carla Ragonese; Peter Quinto Tranchida; Paola Dugo; Giovanni Dugo; Leonard M Sidisky; Mark V Robillard; Luigi Mondello
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical chemistry     Volume:  81     ISSN:  1520-6882     ISO Abbreviation:  Anal. Chem.     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-06-30     Completed Date:  2009-09-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5561-8     Citation Subset:  IM    
Dipartimento Farmaco-Chimico, Facoltà di Farmacia, Università dei Messina, Viale Annunziata, I-98168, Messina, Italy.
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MeSH Terms
Chromatography, Gas / methods*
Evaluation Studies as Topic
Ionic Liquids / chemistry*
Trans Fatty Acids / analysis*
Reg. No./Substance:
0/Ionic Liquids; 0/Trans Fatty Acids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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