Document Detail


Evaluation of the use of conductimetry for the rapid and precise measurement of Salmonella spp. growth rates.
MedLine Citation:
PMID:  16616386     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The growth rates of 14 Salmonella serovars in tryptone soy broth plus yeast extract (TSBYE) were estimated using conventional plating techniques and indirect conductimetry using a Don Whitley RABIT system. Both methods gave identical results for the maximum specific growth rate (mumax) P>0.05. However, using the conductimetric method, mumax for a single serovar was determined in less than 7 h, whereas the conventional method required an additional 24 h. In addition, the conductimetric method was considerably more precise, much less labour-intensive and required the use of considerably less consumables. Using conductimetry, a trained operator could accurately determine mumax for 24 serovars in 3 working days, but only one serovar using the conventional plate counting technique. Hence, the use of conductimetry can markedly increase the precision and accuracy of mumax determinations by allowing a very significant increase in the number of results obtained and in their precision. The data generated will allow the development of better mathematical growth models. The method can also be used to compare growth media and conditions and hence rapidly optimise detection protocols for this pathogen.
Authors:
A E Sherry; M F Patterson; D Kilpatrick; R H Madden
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-04-17
Journal Detail:
Title:  Journal of microbiological methods     Volume:  67     ISSN:  0167-7012     ISO Abbreviation:  J. Microbiol. Methods     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-08-28     Completed Date:  2006-12-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8306883     Medline TA:  J Microbiol Methods     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  86-92     Citation Subset:  IM    
Affiliation:
Food Microbiology, Queen's University of Belfast, Newforge Lane, Belfast BT9 5PX, Northern Ireland, UK.
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MeSH Terms
Descriptor/Qualifier:
Bacteriological Techniques / methods*
Salmonella / growth & development*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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