| Evaluation of two monoclonal antibodies for serotyping Haemophilus paragallinarum. | |
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MedLine Citation:
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PMID: 2282016 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Two monoclonal antibodies (MAbs) were evaluated for their ability to serotype 108 isolates of Haemophilus paragallinarum. One MAb (E5C12D10) was raised against a Page serovar A strain and the other (F2E6) against a Page serovar C strain. In both dot blot and hemagglutination-inhibition tests, MAb E5C12D10 recognized the type strains of Page serovar A and Kume serovars A-1, A-2, A-3, and A-4. MAb F2E6 recognized the type strains of Page serovar C and Kume serovars C-1, C-2, and C-3. Neither antibody recognized the type strains of Page serovar B or Kume serovars B-1 and C-4. When evaluated with 97 field isolates in a dot blot test, the MAbs serotyped 81 isolates, which was better than agglutinin typing by the Page scheme (69 isolates serotyped). The field isolates that did not react with the MAbs were either Page serovar B/Kume serovar B-1 (three isolates), Page serovar C/Kume serovar C-4 (12 isolates), or nontypable by either the Page or Kume scheme (one isolate). |
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Authors:
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P J Blackall; T Yamaguchi; Y Iritani; D G Rogers |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Avian diseases Volume: 34 ISSN: 0005-2086 ISO Abbreviation: Avian Dis. Publication Date: 1990 Oct-Dec |
Date Detail:
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Created Date: 1991-03-14 Completed Date: 1991-03-14 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370617 Medline TA: Avian Dis Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 861-4 Citation Subset: IM |
Affiliation:
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Queensland Department of Primary Industries, Animal Research Institute, Yeerongpilly, Australia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Monoclonal / diagnostic use* Chickens Haemophilus / classification* Hemagglutination Inhibition Tests Immunoblotting Serotyping |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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