| Evaluation of susceptibility testing methods for polymyxin. | |
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MedLine Citation:
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PMID: 20045367 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: The widespread resistance in Gram-negative bacteria has necessitated evaluation of the use of older antimicrobials such as polymyxins. In the present study we evaluated the different susceptibility testing methods for polymyxins B and E against Gram-negative bacteria using the new Clinical and Laboratory Standards Institute (CLSI) guidelines. METHODS: The susceptibility of 281 multidrug-resistant (MDR) Gram-negative bacteria (GNB) to polymyxin B was evaluated, comparing broth microdilution (BMD; reference method), agar dilution, E-test, and disk diffusion. Disk diffusion testing of polymyxin B was also performed against 723 MDR GNB. RESULTS: Twenty-four of 281 (8.5%) isolates were found to be resistant to polymyxin B by the reference BMD method. The rates of very major errors for agar dilution and E-test (for polymyxin B) were 0.7% and 1%, respectively, and those for disk diffusion (for polymyxin B and polymyxin E) were 1% and 0.7%, respectively. For the 257 isolates found sensitive by reference BMD, the rates of major errors by agar dilution and E-test (for polymyxin B) were 2.4% and 0%, respectively, and those for disk diffusion (polymyxin B and polymyxin E) were 0% and 0.7%, respectively. Twenty-six (3.6%) of the 723 Gram-negative isolates were resistant to polymyxin B by disk diffusion. CONCLUSION: The E-test and agar dilution methods showed good concordance with BMD. The disk diffusion method can be useful for initial screening in diagnostic laboratories. |
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Authors:
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Bijayini Behera; Purva Mathur; Anupam Das; Arti Kapil; Babita Gupta; Sanjeev Bhoi; Kamran Farooque; Vijay Sharma; M C Misra |
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Publication Detail:
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Type: Evaluation Studies; Journal Article Date: 2009-12-31 |
Journal Detail:
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Title: International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases Volume: 14 ISSN: 1878-3511 ISO Abbreviation: Int. J. Infect. Dis. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-06-15 Completed Date: 2010-09-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9610933 Medline TA: Int J Infect Dis Country: Canada |
Other Details:
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Languages: eng Pagination: e596-601 Citation Subset: IM |
Copyright Information:
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Copyright 2009 International Society for Infectious Diseases. Published by Elsevier Ltd. All rights reserved. |
Affiliation:
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Department of Laboratory Medicine, Jai Prakash Narain Apex (JPNA) Trauma Centre, All India Institute of Medical Sciences (AIIMS), New Delhi, 110029 India. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Anti-Bacterial Agents
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pharmacology* Colistin / pharmacology* Disk Diffusion Antimicrobial Tests / methods, standards Drug Resistance, Bacterial Gram-Negative Bacteria / drug effects* Humans Microbial Sensitivity Tests* / methods, standards Polymyxin B / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Anti-Bacterial Agents; 1066-17-7/Colistin; 1404-26-8/Polymyxin B |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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