Document Detail


Evaluation of suitable reference gene for real-time PCR in human umbilical cord mesenchymal stem cells with long-term in vitro expansion.
MedLine Citation:
PMID:  20440577     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In real-time quantitative PCR, the accuracy of normalized data is highly dependent on the stability of the reference genes. However, reference gene expression in a given cell type or experimental condition can vary considerably. The goal of this study was to establish a reliable set of reference genes for real-time PCR studies using human umbilical cord mesenchymal stem cells with long-term in vitro expansion. The stability of ten potential reference genes was examined in human umbilical cord mesenchymal stem cells. We found that Ywhaz and Rpl13a, not beta-actin or Gapdh, were the most stably expressed of the internal control genes in different passages of human umbilical cord mesenchymal stem cells. Ywhaz and Rpl13a could be used as reference genes for relative gene quantification and normalization purposes in real-time PCR studies of human umbilical cord mesenchymal stem cells.
Authors:
Youwei Wang; Zhibo Han; Shulin Yan; Aibin Mao; Bin Wang; He Ren; Ying Chi; Zhongchao Han
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-05-04
Journal Detail:
Title:  In vitro cellular & developmental biology. Animal     Volume:  46     ISSN:  1543-706X     ISO Abbreviation:  In Vitro Cell. Dev. Biol. Anim.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-07-08     Completed Date:  2010-11-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9418515     Medline TA:  In Vitro Cell Dev Biol Anim     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  595-9     Citation Subset:  IM    
Affiliation:
National Engineering Research Center of Cell Products, AmCellGene Co. Ltd, TEDA, Tianjin 300457, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Cell Differentiation
Cell Proliferation
Cell Separation
Cell Shape
Cells, Cultured
Gene Expression Regulation
Genes / genetics*
Humans
Mesenchymal Stem Cells / cytology*,  metabolism*
Reference Standards
Reverse Transcriptase Polymerase Chain Reaction / methods*
Software
Time Factors
Umbilical Cord / cytology*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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