Document Detail

Evaluation of study design variables and their impact on food-maintained operant responding in mice.
MedLine Citation:
PMID:  19879302     Owner:  NLM     Status:  MEDLINE    
Operant conditioning paradigms are useful for studying factors involved in reward, particularly when combined with the tools of genetic manipulation in mice. Published operant studies involving mice vary widely with respect to design, and insight into the consequences of design choices on performance in mice is limited. Here, we evaluated the impact of five design variables on the performance of inbred male mice in operant tasks involving solid food pellets as reinforcing agents. We found that the use of lever-press or nose-poke during FR1 sessions did not impact the performance of C57BL/6 mice, but that the lever-press approach correlated with enhanced performance during PR testing. While FR1 session duration had a notable impact on the rate of acquisition of food-maintained responding, performance during FR1 and PR sessions was largely unaffected. Higher order schedules of reinforcement (FR3 and FR5) led to elevated responding during both FR and PR sessions, and improved the correspondence between rewards earned and consumed. Single and group-housed mice performed indistinguishably during FR1 and PR sessions, while environmental enrichment combined with group housing accelerated the rate of acquisition of food-maintained responding while decreasing responding during PR testing. Finally, while C57BL/6 and 129/Sv mice exhibited comparable behavior during FR1 sessions, C57BL/6 mice tended to acquire food-maintained responding faster than 129/Sv counterparts, and exhibited elevated responding during PR testing. Altogether, our findings indicate that while operant performance for food in mice is relatively insensitive to many study parameters, experimental outcomes can be shaped predictably with proper design decisions.
Desirae M Haluk; Kevin Wickman
Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural     Date:  2009-10-29
Journal Detail:
Title:  Behavioural brain research     Volume:  207     ISSN:  1872-7549     ISO Abbreviation:  Behav. Brain Res.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-02-01     Completed Date:  2010-04-13     Revised Date:  2014-09-19    
Medline Journal Info:
Nlm Unique ID:  8004872     Medline TA:  Behav Brain Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  394-401     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Elsevier B.V. All rights reserved.
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MeSH Terms
Biomedical Research / methods*
Conditioning, Operant*
Housing, Animal
Mice, Inbred C57BL
Mice, Inbred Strains
Random Allocation
Reinforcement Schedule
Species Specificity
Time Factors
Grant Support
DA011806/DA/NIDA NIH HHS; MH061933/MH/NIMH NIH HHS; P50 DA011806/DA/NIDA NIH HHS; P50 DA011806-120015/DA/NIDA NIH HHS; R01 MH061933/MH/NIMH NIH HHS; R01 MH061933-08/MH/NIMH NIH HHS

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