Document Detail


Evaluation of structure and hydrolysis activity of Candida rugosa Lip7 in presence of sub-/super-critical CO(2).
MedLine Citation:
PMID:  23040391     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
This work aimed to assess the effect of sub-/super-critical CO(2) on the structure and activity of Candida rugosa Lip7 (CRL7) in its solution form. The structure was examined by SDS-PAGE gel electrophoresis, circular dichroism (CD) and fluorescence spectra photometry. Results revealed that the primary structure remained intact after sub-/super-critical CO(2) treatment, and the secondary structure altered at the pressure of 10MPa and temperature 40°C for 30min incubation, but it was reflex to its native form with increasing incubation time up to 150min under 10MPa and 40°C. Meanwhile, the tertiary structure via fluorescence spectra analysis showed that the intensity of the maximal emission wavelength at 338nm decreased under the conditions of 10MPa and 40°C for 150min. Furthermore, the residue hydrolysis activity and kinetics constants (V(max) and K(m)) of CRL7 treated with sub-/super-critical CO(2) were also investigated. In cases of 6MPa and 35°C, or 10MPa and 40°C for 30min, activity variance of CRL7 was maybe caused by its secondary structure alteration. But in case of 10MPa and 40°C for 150min, the tertiary structure change was perhaps responsibility for CRL7 activity enhancement.
Authors:
Yun Liu; Dawei Chen; Li Xu; Yunjun Yan
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-16
Journal Detail:
Title:  Enzyme and microbial technology     Volume:  51     ISSN:  1879-0909     ISO Abbreviation:  Enzyme Microb. Technol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-10-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8003761     Medline TA:  Enzyme Microb Technol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  354-358     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier Inc. All rights reserved.
Affiliation:
Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China; State Key Lab of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China; Key Laboratory for Green Chemical Process of Ministry of Education, Wuhan Institute of Technology, Wuhan 430073, China; College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China. Electronic address: liuyunprivate@sian.com.
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