Document Detail


Evaluation of stable isotope fingerprinting techniques for the assessment of the predominant methanogenic pathways in anaerobic digesters.
MedLine Citation:
PMID:  23299457     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Laboratory biogas reactors were operated under various conditions using maize silage, chicken manure, or distillers grains as substrate. In addition to the standard process parameters, the hydrogen and carbon stable isotopic composition of biogas was analyzed to estimate the predominant methanogenic pathways as a potential process control tool. The isotopic fingerprinting technique was evaluated by parallel analysis of mcrA genes and their transcripts to study the diversity and activity of methanogens. The dominant hydrogenotrophs were Methanomicrobiales, while aceticlastic methanogens were represented by Methanosaeta and Methanosarcina at low and high organic loading rates, respectively. Major changes in the relative abundance of mcrA transcripts were observed compared to the results obtained from DNA level. In agreement with the molecular results, the isotope data suggested the predominance of the hydrogenotrophic pathway in one reactor fed with chicken manure, while both pathways were important in the other reactors. Short-term changes in the isotopic composition were followed, and a significant change in isotope values was observed after feeding a reactor digesting maize silage. This ability of stable isotope fingerprinting to follow short-term activity changes shows potential for indicating process failures and makes it a promising technology for process control.
Authors:
M Nikolausz; R F H Walter; H Sträuber; J Liebetrau; T Schmidt; S Kleinsteuber; F Bratfisch; U Günther; H H Richnow
Related Documents :
24178367 - Influence of thiourea application on some physiological and molecular criteria of sunfl...
24142387 - Comparative analysis of different biofactories for the production of a major diabetes a...
23331977 - Calcineurin b-like protein cbl10 directly interacts with akt1 and modulates k(+) homeos...
15943967 - Plant cullin-based e3s: phytohormones come first.
16382957 - Mineralogy of air-pollution-control residues from a secondary lead smelter: environment...
20929897 - Auxin transport in maize roots in response to localized nitrate supply.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-10
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  -     ISSN:  1432-0614     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Bioenergy, UFZ-Helmholtz Centre for Environmental Research, Permoserstr. 15, 04318, Leipzig, Germany, marcell.nikolausz@ufz.de.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A family 5 ?-mannanase from the thermophilic fungus Thielavia arenaria XZ7 with typical thermophilic...
Next Document:  The intracellular galactoglycome in Trichoderma reesei during growth on lactose.