Document Detail

Evaluation of single-cell sources of docosahexaenoic acid and arachidonic acid: 3-month rat oral safety study with an in utero phase.
MedLine Citation:
PMID:  10069479     Owner:  NLM     Status:  MEDLINE    
Owing to the presence of the polyunsaturated fatty acids (PUFA) docosahexaenoic acid (DHA) and arachidonic acid (ARA) in human milk and their important biological function, several authorities recommend that they be added to infant formulas. This study assessed the safety of an algal oil rich in DHA and a fungal oil rich in ARA, blended to provide a DHA to ARA ratio similar to human milk. The oil blend was incorporated into diets and fed to rats such that they received 3, 11 and 22 times the anticipated infant exposure to DHA and ARA. Low-fat and high-fat control groups received canola oil. Rats received experimental diets over a premating interval and throughout mating, gestation and lactation. Pups born during this period (F1) consumed treatment diets from weaning for 3 months. Physical observations, ophthalmoscopic examinations, body weight, food intake, clinical chemistry, neurobehavioural evaluations and postmortem histopathology of selected tissues were performed. No statistically significant, dose-dependent adverse effects were seen in reproductive performance or fertility, nor in the neonates from birth to weaning. Mid- and high-dose treated F1 animals exhibited increased white cell count, neutrophil count and blood urea nitrogen; increased liver and spleen weights (absolute and relative to body weight) also were observed. There were no corresponding microscopic findings. The clinical pathology and organ weight differences at these treatment levels represent physiological or metabolic responses to the test substance rather than adverse responses. These single-cell oils produced no adverse effects in rats when administered in utero and for 90 days at dietary levels resulting in exposures up to 22 or 66 times higher than those expected in infant formulas when extrapolated on the basis of diet composition (g/100 Cal) or intake (g/kg body weight), respectively.
R A Burns; G J Wibert; D A Diersen-Schade; C M Kelly
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association     Volume:  37     ISSN:  0278-6915     ISO Abbreviation:  Food Chem. Toxicol.     Publication Date:  1999 Jan 
Date Detail:
Created Date:  1999-03-18     Completed Date:  1999-03-18     Revised Date:  2006-03-13    
Medline Journal Info:
Nlm Unique ID:  8207483     Medline TA:  Food Chem Toxicol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  23-36     Citation Subset:  IM    
Mead Johnson Research Center, Evansville, IN 47721, USA.
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MeSH Terms
Administration, Oral
Animals, Newborn
Animals, Suckling
Arachidonic Acid / administration & dosage,  toxicity*
Body Weight / drug effects*
Dinoflagellida / chemistry
Docosahexaenoic Acids / administration & dosage,  toxicity*
Infant Food* / analysis,  toxicity
Infant, Newborn
Milk, Human / chemistry
Mortierella / chemistry
Organ Size / drug effects
Random Allocation
Rats, Sprague-Dawley
Reproduction / drug effects*
Reg. No./Substance:
25167-62-8/Docosahexaenoic Acids; 506-32-1/Arachidonic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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