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Evaluation of single-cell gel electrophoresis data: Combination of variance analysis with sum of ranking differences.
MedLine Citation:
PMID:  25308437     Owner:  NLM     Status:  In-Data-Review    
Specimens of the mussel Mytilus galloprovincialis were collected from five sites in the Boka Kotorska Bay (Adriatic Sea, Montenegro) during the period summer 2011-autumn 2012. Three types of tissue, haemolymph, digestive gland were used for assessment of DNA damage. Images of randomly selected cells were analyzed with a fluorescence microscope and image analysis by the Comet Assay IV Image-analysis system. Three parameters, viz. tail length, tail intensity and Olive tail moment were analyzed on 4200 nuclei per cell type. We observed variations in the level of DNA damage in mussels collected at different sites, as well as seasonal variations in response. Sum of ranking differences (SRD) was implemented to compare use of different types of cell and different measure of comet tail per nucleus. Numerical scales were transferred into ranks, range scaling between 0 and 1; standardization and normalization were carried out. SRD selected the best (and worst) combinations: tail moment is the best for all data treatment and for all organs; second best is tail length, and intensity ranks third (except for digestive gland). The differences were significant at the 5% level. Whereas gills and haemolymph cells do not differ significantly, cells of the digestive gland are much more suitable to estimate genotoxicity. Variance analysis decomposed the effect of different factors on the SRD values. This unique combination has provided not only the relative importance of factors, but also an overall evaluation: the best evaluation method, the best data pre-treatment, etc., were chosen even for partially contradictory data. The rank transformation is superior to any other way of scaling, which is proven by ordering the SRD values by SRD again, and by cross validation.
Károly Héberger; Stoimir Kolarević; Margareta Kračun-Kolarević; Karolina Sunjog; Zoran Gačić; Zoran Kljajić; Milena Mitrić; Branka Vuković-Gačić
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Publication Detail:
Type:  Journal Article     Date:  2014-06-21
Journal Detail:
Title:  Mutation research. Genetic toxicology and environmental mutagenesis     Volume:  771     ISSN:  1879-3592     ISO Abbreviation:  Mutat Res Genet Toxicol Environ Mutagen     Publication Date:  2014 Sep 
Date Detail:
Created Date:  2014-10-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101632149     Medline TA:  Mutat Res Genet Toxicol Environ Mutagen     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  15-22     Citation Subset:  IM    
Copyright Information:
Copyright © 2014 Elsevier B.V. All rights reserved.
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