Document Detail


Evaluation of serum-free differentiation conditions for C2C12 myoblast cells assessed as to active tension generation capability.
MedLine Citation:
PMID:  20635352     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have compared several serum-free media for the differentiation of C2C12 myoblasts and assessed the extent of differentiation in several ways including as to active tension generation capability. C2C12 cells were allowed to differentiate in Dulbecco's modified Eagle's medium (DMEM) containing Ham's F-12 (F-12), AIM-V (AIM), 0.2% Ultroser-G in DMEM (Ult-G), and 0.1% Sericin in DMEM (Sericin), compared with in DMEM supplemented with 2% horse serum (HS) or 2% calf serum (CS). C2C12 differentiation was assessed as the extent of myotube formation, glucose metabolism, protein expression, sarcomere formation, and active tension generation. All serum-free media examined were capable of inducing myotube formation and the expression of muscle-specific proteins. All serum-free media except for F-12 gave the sarcomere structure. Active tension generation was observed for cells that differentiated in AIM and Ult-G, but the active tension generated by C2C12 cells that differentiated in Ult-G was only ∼25% in the case of myotubes that formed in HS. The addition of Ult-G to the AIM resulted in improvement of the active tension generation capability, the active tension generated being ∼3.4× compared to that in HS. The approach for assessing muscle cell differentiation presented in this study will be suitable for other studies that involve the differentiation of muscle cells.
Authors:
Hideaki Fujita; Akiko Endo; Kazunori Shimizu; Eiji Nagamori
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biotechnology and bioengineering     Volume:  107     ISSN:  1097-0290     ISO Abbreviation:  Biotechnol. Bioeng.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-10-25     Completed Date:  2011-01-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7502021     Medline TA:  Biotechnol Bioeng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  894-901     Citation Subset:  IM    
Copyright Information:
© 2010 Wiley Periodicals, Inc.
Affiliation:
Toyota Central R&D Labs, Inc., 41-1 Yokomichi, Nagakute, Aichi 480-1192, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Differentiation
Culture Media / chemistry
Culture Media, Serum-Free
Mice
Muscle Fibers, Skeletal / physiology
Muscle Tonus*
Myoblasts / physiology*
Chemical
Reg. No./Substance:
0/Culture Media; 0/Culture Media, Serum-Free

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