| Evaluation of sequential perfusion with Euro-Collins and Belzer solutions for pancreas preservation in rats. | |
| | |
MedLine Citation:
|
PMID: 17112828 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
OBJECTIVE: To investigate the effect of Euro-Collins and Belzer solutions in a sequential preservation of the pancreas. METHODS: Forty-five Wistar-EPM rats were divided into four groups, according to the solution used during preservation: (1) saline solution (SF): animals perfused and preserved with saline solution; (2) Euro-Collins group (C): animals perfused and preserved with Euro-Collins solution; (3) Belzer group (B): animals perfused and preserved with Belzer solution; (4) Euro-Collins/Belzer group (CB): animals perfused with equal parts of Euro-Collins and Belzer solutions sequentially and preserved with Belzer solution. After perfusion, the animals underwent pancreas resection and preservation with the respective substance at 4 degrees C. Amylase was measured in the preservation solution after 12, 24, 36, or 48 hours. Finally, the pancreas was analyzed histologically, and a statistical analysis was performed. RESULTS: Groups SF and C showed the highest amylase levels in the preservation solution during all periods. The levels were higher than in groups C and CB (P = .05). Amylase levels were similar in groups B and CB to 24 hours (P = .05). Histological analysis was significant for analysis of pancreas islet cells and edema. Groups B and CB were histologically similar (P = .001) and different from groups SF and C. CONCLUSION: Sequential perfusion using Euro-Collins and Belzer solutions was effective for pancreas preservation in rats up to 24 hours. |
| | |
Authors:
|
V Schraibman; A Goldenberg; I Hong Jun Koh; A Miziara Gonzalez; A Molinari; E Cezarino de Morais; R Artigiani; D Matos |
Related Documents
:
|
2559068 - Cgmp immunocytochemistry in aorta, kidney, retina and brain tissues of the rat after pe... 1215848 - Rubidium redistribution after suprarenal caval ligation in the rat. 21167958 - Prostacyclin, not only nitric oxide, is a mediator of the vasorelaxation induced by ace... 285708 - The effect of insulin and hydrocortisone on the isolated rat liver. 325978 - Effect of prolactin inhibitory agents on the ectopic anterior pituitary and the mammary... 1812088 - Is there an inter-organ glutathione redox cycle? |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Transplantation proceedings Volume: 38 ISSN: 0041-1345 ISO Abbreviation: Transplant. Proc. Publication Date: 2006 Nov |
Date Detail:
|
Created Date: 2006-11-20 Completed Date: 2007-02-12 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0243532 Medline TA: Transplant Proc Country: United States |
Other Details:
|
Languages: eng Pagination: 2780-3 Citation Subset: IM |
Affiliation:
|
Gastric Surgery Section, Department of Surgery, Federal University of São Paulo, Sao Paulo, Brazil. vschraibman@hotmail.com |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adenosine
/
pharmacology Allopurinol / pharmacology Animals Glutathione / pharmacology Hypertonic Solutions* / pharmacology Inflammation Insulin / pharmacology Models, Animal Organ Preservation / methods* Organ Preservation Solutions* / pharmacology Pancreas* / drug effects, physiopathology Raffinose / pharmacology Rats Rats, Wistar Time Factors |
| Chemical | |
Reg. No./Substance:
|
0/Euro-Collins' solution; 0/Hypertonic Solutions; 0/Organ Preservation Solutions; 0/University of Wisconsin-lactobionate solution; 11061-68-0/Insulin; 315-30-0/Allopurinol; 512-69-6/Raffinose; 58-61-7/Adenosine; 70-18-8/Glutathione |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Ulinastatin attenuates lung ischemia-reperfusion injury in rats by inhibiting tumor necrosis factor ...
Next Document: Expression of antiapoptotic survivin and aven genes in rat heart tissue after traumatic brain injury...