Document Detail

Evaluation of rhodamine B as an orally delivered biomarker for rodents and a feed-through transtadial biomarker for phlebotomine sand flies (Diptera: Psychodidae).
MedLine Citation:
PMID:  19769045     Owner:  NLM     Status:  MEDLINE    
The purpose of this study was to evaluate the use of rhodamine B as an orally delivered biomarker for rodents and a feed-through transtadial biomarker for phlebotomine sand flies (Diptera: Psychodidae). Rhodamine B-treated hamsters were visibly marked for up to 8 wk, and their feces were fluorescent when examined under a fluorescence microscope. The development and survival of sand fly larvae fed feces of rhodamine B-treated hamsters were not significantly different from control sand flies. Adult male and female sand flies, that had been fed as larvae the feces of rhodamine B-treated hamsters, were fluorescent when examined using fluorescent microscopy and could be distinguished from control sand flies. Adult female sand flies that took bloodmeals from rhodamine B-treated hamsters were fluorescent when examined immediately after feeding. Rhodamine B incorporated rodent baits could be used to detect adult male and female sand flies that fed on the feces of baited rodents as larvae, or adult female sand flies that have taken a bloodmeal from bait-fed rodents. This would allow the delineation of specific foci with rodent-sand fly associations that would be susceptible to control by using feed-through or systemic insecticides.
T M Mascari; L D Foil
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of medical entomology     Volume:  46     ISSN:  0022-2585     ISO Abbreviation:  J. Med. Entomol.     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-09-22     Completed Date:  2009-10-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0375400     Medline TA:  J Med Entomol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1131-7     Citation Subset:  IM    
Department of Entomology, Louisiana State University Agricultural Center, Agricultural Experiment Station, 402 Life Sciences, Baton Rouge, LA 70803, USA.
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MeSH Terms
Administration, Oral
Feces / chemistry*
Feeding Behavior
Fluorescent Dyes / administration & dosage,  analysis*
Larva / chemistry
Phlebotomus / chemistry*
Rhodamines / administration & dosage,  analysis*
Reg. No./Substance:
0/Fluorescent Dyes; 0/Rhodamines; 14899-08-2/rhodamine B

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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