Document Detail


Evaluation of a revised u.s. Food and drug administration method for the detection of cronobacter in powdered infant formula: a collaborative study.
MedLine Citation:
PMID:  22691486     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
A revised U.S. Food and Drug Administration (FDA) method for the isolation and detection of Cronobacter from powdered infant formula was recently developed, which combines real-time PCR, chromogenic agars, and RAPID ID 32E biochemical tests. This method provides an expedient analysis within 24 to 48 h. A collaborative validation study involving four different laboratories was conducted to compare the revised FDA method with the reference FDA method using casein- and soy-based powdered infant formula inoculated with different Cronobacter strains. Valid results from 216 test portions and controls from collaborating laboratories were obtained and showed that the revised FDA method performed significantly better than the reference FDA method. Newly revised PCR protocols and VITEK 2 were also evaluated to be integrated into the complete detection procedure.
Authors:
Yi Chen; Kathy E Noe; Sandra Thompson; Carol A Elems; Eric W Brown; Keith A Lampel; Thomas S Hammack
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of food protection     Volume:  75     ISSN:  1944-9097     ISO Abbreviation:  J. Food Prot.     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-06-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1144-7     Citation Subset:  IM    
Affiliation:
Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, 5100 Paint Branch Parkway, College Park, Maryland 20740, USA. yi.chen@fda.hhs.gov.
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