| Evaluation of the reversed passive latex agglutination (RPLA) test kits for detection of staphylococcal enterotoxins A, B, C, and D in foods. | |
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MedLine Citation:
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PMID: 3779524 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Four lots of the SET-RPLA kit (Denka Seiken Co. Ltd., Tokyo), a commercial reverse passive latex agglutination test kit for the detection of staphylococcal enterotoxins A, B, C, and D in foods, have been evaluated for their efficacy. The kits showed high specificity and sensitivity with a detection limit of 0.75 ng enterotoxin/g of food. The test is simple, is completed within 24 h, and does not require complicated extraction or concentration procedures nor expensive equipment. In addition, the assay is semiquantitative. However, as in any other immunological system, routine verification of the specificity of the latex reagents against standard enterotoxins and toxin-free food extracts is necessary. |
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Authors:
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C E Park; R Szabo |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Canadian journal of microbiology Volume: 32 ISSN: 0008-4166 ISO Abbreviation: Can. J. Microbiol. Publication Date: 1986 Sep |
Date Detail:
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Created Date: 1987-01-21 Completed Date: 1987-01-21 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372707 Medline TA: Can J Microbiol Country: CANADA |
Other Details:
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Languages: eng Pagination: 723-7 Citation Subset: IM |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Enterotoxins
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analysis* Evaluation Studies as Topic Food Analysis Food Microbiology* Latex Fixation Tests / methods Reagent Kits, Diagnostic |
| Chemical | |
Reg. No./Substance:
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0/Enterotoxins; 0/Reagent Kits, Diagnostic; 12788-99-7/enterotoxin D, Staphylococcal; 37337-57-8/enterotoxin A, Staphylococcal; 39424-53-8/enterotoxin B, staphylococcal; 39424-54-9/enterotoxin C, staphylococcal |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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