Document Detail

Evaluation of the reversed passive latex agglutination (RPLA) test kits for detection of staphylococcal enterotoxins A, B, C, and D in foods.
MedLine Citation:
PMID:  3779524     Owner:  NLM     Status:  MEDLINE    
Four lots of the SET-RPLA kit (Denka Seiken Co. Ltd., Tokyo), a commercial reverse passive latex agglutination test kit for the detection of staphylococcal enterotoxins A, B, C, and D in foods, have been evaluated for their efficacy. The kits showed high specificity and sensitivity with a detection limit of 0.75 ng enterotoxin/g of food. The test is simple, is completed within 24 h, and does not require complicated extraction or concentration procedures nor expensive equipment. In addition, the assay is semiquantitative. However, as in any other immunological system, routine verification of the specificity of the latex reagents against standard enterotoxins and toxin-free food extracts is necessary.
C E Park; R Szabo
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Canadian journal of microbiology     Volume:  32     ISSN:  0008-4166     ISO Abbreviation:  Can. J. Microbiol.     Publication Date:  1986 Sep 
Date Detail:
Created Date:  1987-01-21     Completed Date:  1987-01-21     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0372707     Medline TA:  Can J Microbiol     Country:  CANADA    
Other Details:
Languages:  eng     Pagination:  723-7     Citation Subset:  IM    
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MeSH Terms
Enterotoxins / analysis*
Evaluation Studies as Topic
Food Analysis
Food Microbiology*
Latex Fixation Tests / methods
Reagent Kits, Diagnostic
Reg. No./Substance:
0/Enterotoxins; 0/Reagent Kits, Diagnostic; 12788-99-7/enterotoxin D, Staphylococcal; 37337-57-8/enterotoxin A, Staphylococcal; 39424-53-8/enterotoxin B, staphylococcal; 39424-54-9/enterotoxin C, staphylococcal

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